CD47 antibody

Catalog No. ABIN6296670

This anti-CD47 antibody is a Mouse Monoclonal antibody detecting CD47 in FACS, WB, IHC, IF, Func and BP. Suitable for Human.

Quick Overview for CD47 antibody (ABIN6296670)

Target: CD47

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD47 antibody is un-conjugated

Application: Flow Cytometry (FACS), Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Functional Studies (Func), Blocking Peptide (BP)

Product Details anti-CD47 Antibody

Purpose: Mouse anti-Human CD47 Antibody [Sodium Azide Free]

Specificity: Cell surface, cytoplasmic

Purification: Intact CD47 purified from placenta was used as the immunogen for this antibody.

Immunogen: Intact CD47 purified from placenta was used as the immunogen for this antibody.

Application Details

Application Notes: Functional Activity (Blocking): Order BSA/Azide-free format
FACS: 0.5-1 μg/million cells
IF: 1-2 μg/mL
IHC (FFPE): 0.5-1 μg/mL for 30 min at RT
Western blot: 2-5 μg/mL

Restrictions: For Research Use only

Handling

Buffer: In 1X PBS ( pH 7.4), BSA free, sodium azide free

Preservative: Azide free

Storage: 4 °C,-20 °C

Storage Comment: 2-8°C. The azide-free format should be aliquoted and stored at -20°C or colder.

Target Details for CD47

Target: CD47

Alternative Name: Leukocyte surface antigen CD47

Background:

Target Description: This antibody reacts with the Ig domain of CD47 protein. It has been shown to inhibit polymorphonuclear neutrophil (PMN) transmigration across cell monolayers and matrix. CD47, originally named integrin-associated protein (IAP), is a 50 kDa protein containing five membrane-spanning sequences and a short cytoplasmic tail. CD47 plays a role in both cell adhesion by acting as an adhesion receptor for THBS1 on platelets, and in the modulation of integrins. It is important in memory formation and synaptic plasticity in the hippocampus. CD47 may play a role in membrane transport and/or integrin dependent signal transduction.

Gene Symbol: CD47///MER6

Gene ID: 961

UniProt: Q08722