beta-2 Microglobulin antibody

Catalog No. ABIN6296700

The Mouse Monoclonal anti-beta-2 Microglobulin antibody (ABIN6296700) specifically detects beta-2 Microglobulin in FACS, IF, IP and IHC (fro). The antibody is reactive with Human and Non-Human Primate samples.

Quick Overview for beta-2 Microglobulin antibody (ABIN6296700)

Target: beta-2 Microglobulin (B2M) (beta-2-Microglobulin (B2M))

Reactivity: Human, Non-Human Primate

Host: Mouse

Clonality: Monoclonal

Conjugate: This beta-2 Microglobulin antibody is un-conjugated

Application: Flow Cytometry (FACS), Immunofluorescence (IF), Immunoprecipitation (IP), Immunohistochemistry (Frozen Sections) (IHC (fro))

Product Details anti-beta-2 Microglobulin Antibody

Purpose: Mouse anti-Human/non-Human primates Beta-2 Microglobulin Antibody [Sodium Azide Free]

Specificity: Cytoplasmic

Purification: MOLT-4 human T cell line was used as the immunogen for the Beta-2 Microglobulin antibody.

Immunogen: MOLT-4 human T cell line was used as the immunogen for the Beta-2 Microglobulin antibody.

Application Details

Application Notes: Flow Cytometry: 0.5-1 μg/million cells in 0.1ml
Immunofluorescence: 0.5-1 μg/mL
Immunoprecipitation: 2 μg/mg of lysate
Immunohistochemistry (Frozen): 0.5-1 μg/mL for 30 min at RT

Restrictions: For Research Use only

Handling

Buffer: In 1X PBS, BSA free, sodium azide free

Preservative: Azide free

Storage: 4 °C,-20 °C

Storage Comment: 2-8°C. The azide-free format should be aliquoted and stored at -20°C or colder.

Target Details for beta-2 Microglobulin

Target: beta-2 Microglobulin (B2M) (beta-2-Microglobulin (B2M))

Alternative Name: Beta-2-microglobulin

Background:

Target Description: Recognizes a protein of 12 kDa, identified as microglobulin. Major histocompatibility complex (MHC) class 1 Molecules bind to antigens for presentation on the surface of cells. The proteasome is responsible for producing these antigens from the components of foreign pathogens. MHC class 1 Molecules consist of an alpha- heavy chain that contains three subdomains (alpha-1, alpha-2, alpha-3) and a non-covalent associating light chain, known as beta-2-Microglobulin. Beta-2-Microglobulin associates with the alpha-3 subdomain of the alpha- heavy chain and forms an immunoglobulin domain-like structure that mediates proper folding and expression of MHC class 1 Molecules. The alpha-1 and alpha-2 domains of the alpha- heavy chain form the peptide antigen-binding cleft. Mutations in the beta-2-Microglobulin gene can enhance the progression of malignant melanoma phenotypes.

Gene Symbol: B2M///CDABP0092///HDCMA22P

Gene ID: 567

UniProt: P61769

Pathways: TCR Signaling, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process