SEMA4D/CD100 antibody

Catalog No. ABIN6296786

The Mouse Monoclonal anti-SEMA4D/CD100 antibody is suitable to detect SEMA4D/CD100 in samples from Human, Mouse and Monkey. It has been validated for FACS, IF and Func.

Quick Overview for SEMA4D/CD100 antibody (ABIN6296786)

Target: SEMA4D/CD100 (SEMA4D) (Sema Domain, Immunoglobulin Domain (Ig), Transmembrane Domain (TM) and Short Cytoplasmic Domain, (Semaphorin) 4D (SEMA4D))

Reactivity: Human, Mouse, Monkey

Host: Mouse

Clonality: Monoclonal

Conjugate: This SEMA4D/CD100 antibody is un-conjugated

Application: Flow Cytometry (FACS), Immunofluorescence (IF), Functional Studies (Func)

Product Details anti-SEMA4D/CD100 Antibody

Purpose: Mouse anti-Human/Monkey/Mouse Semaphorin-4D Antibody [Sodium Azide Free]

Specificity: Cell surface

Purification: PHA stimulated human peripheral blood lymphocytes were used as the immunogen for the Semaphorin-4D antibody.

Immunogen: PHA stimulated human peripheral blood lymphocytes were used as the immunogen for the Semaphorin-4D antibody.

Application Details

Application Notes: Functional Activity (Order SAF formulation)
Flow Cytometry: 0.5-1 μg/million cells in 0.1ml
Immunofluorescence: 0.5-1 μg/mL

Restrictions: For Research Use only

Handling

Buffer: In 1X PBS, BSA free, sodium azide free

Preservative: Azide free

Storage: 4 °C,-20 °C

Storage Comment: 2-8°C. The azide-free format should be aliquoted and stored at -20°C or colder.

Target Details for SEMA4D/CD100

Target: SEMA4D/CD100 (SEMA4D) (Sema Domain, Immunoglobulin Domain (Ig), Transmembrane Domain (TM) and Short Cytoplasmic Domain, (Semaphorin) 4D (SEMA4D))

Alternative Name: Semaphorin-4D

Background:

Target Description: Recognizes a homodimeric protein comprised of 50 kDa subunits, identified as CD100. It is expressed on majority of haemopoietic cells (B, T, NK and myeloid cells) and is absent from bone marrow, erythrocytes, eosinophils and endothelial cells. Its expression is increased after PHA-activation. CD100 was shown to associate with different partner molecules in T cells such as CD45, a key molecule with protein tyrosine phosphatase activity involved in T-cell transduction, and a Serine kinase. It plays a role in homotypic cell adhesion and in T cell activation.

Gene Symbol: SEMA4D///C9orf164///CD100///SEMAJ

Gene ID: 10507

UniProt: Q92854

Pathways: Regulation of Cell Size