SUMO1 antibody

Catalog No. ABIN6297475

This Mouse Monoclonal antibody specifically detects SUMO1 in WB, IHC, IF and FACS. It exhibits reactivity toward Human and Rat.

Quick Overview for SUMO1 antibody (ABIN6297475)

Target: SUMO1 (Small Ubiquitin Related Modifier Protein 1 (SUMO1))

Reactivity: Human, Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: This SUMO1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS)

Product Details anti-SUMO1 Antibody

Purpose: Mouse anti-Human/Rat SUMO-1 Antibody (SENP2) [Sodium Azide Free]

Specificity: Predominantly nuclear with some cytoplasmic

Purification: Recombinant human protein was used as the immunogen for the SUMO-1 antibody.

Immunogen: Recombinant human protein was used as the immunogen for the SUMO-1 antibody.

Application Details

Application Notes: Flow Cytometry: 0.5-1 μg/million cells in 0.1ml
Immunofluorescence: 0.5-1 μg/mL
Western blot: 0.5-1 μg/mL
Immunohistochemistry (FFPE): 0.5-1 μg/mL for 30 min at RT (1)
Prediluted format: incubate for 30 min at RT (2)

Restrictions: For Research Use only

Handling

Buffer: In 1X PBS, BSA free, sodium azide free

Preservative: Azide free

Storage: 4 °C,-20 °C

Storage Comment: 2-8°C. The azide-free format should be aliquoted and stored at -20°C or colder.

Target Details for SUMO1

Target: SUMO1 (Small Ubiquitin Related Modifier Protein 1 (SUMO1))

Alternative Name: Small ubiquitin-related modifier 1

Background:

Target Description: This mAb is specific to SUMO-1 and shows no cross-reaction with either SUMO-2 or SUMO-3. The small ubiquitin-related modifier (SUMO) proteins, which include SUMO-1, SUMO-2 and SUMO-3, belong to the ubiquitin-like protein family. Like ubiquitin, the SUMO proteins are synthesized as precursor proteins that undergo processing before conjugation to target proteins. Also, both utilize the E1, E2, and E3 cascade enzymes for conjugation. However, SUMO and ubiquitin differ with respect to targeting. Ubiquitination predominantly targets proteins for degradation, whereas sumoylation targets proteins to a variety of cellular processing, including nuclear transport, transcriptional regulation, apoptosis and protein stability. The unconjugated SUMO-1 protein localizes to the nuclear membrane.

Gene Symbol: SUMO1///SMT3C///SMT3H3///UBL1///OK///SW-cl.43

Gene ID: 7341

UniProt: P63165

Pathways: M Phase, Positive Regulation of Endopeptidase Activity, Protein targeting to Nucleus, Ubiquitin Proteasome Pathway