TLR4 antibody

Catalog No. ABIN6297482

The Mouse Monoclonal anti-TLR4 antibody is suitable to detect TLR4 in samples from Human, Rat, Dog, Pig, Guinea Pig and Monkey. It has been validated for FACS, IHC, IF and Func.

Quick Overview for TLR4 antibody (ABIN6297482)

Target: TLR4 (Toll-Like Receptor 4 (TLR4))

Reactivity: Human, Rat, Dog, Pig, Guinea Pig, Monkey

Host: Mouse

Clonality: Monoclonal

Conjugate: This TLR4 antibody is un-conjugated

Application: Flow Cytometry (FACS), Immunohistochemistry (IHC), Immunofluorescence (IF), Functional Studies (Func)

Product Details anti-TLR4 Antibody

Purpose: Mouse anti-Human/Monkey/Pig/Dog/Rat/Guinea Pig TLR4 Antibody (Toll Like Receptor 4) [Sodium Azide Free]

Specificity: Cell surface

Purification: Recombinant human protein was used as the immunogen for the TLR4 antibody.

Immunogen: Recombinant human protein was used as the immunogen for the TLR4 antibody.

Application Details

Application Notes: Functional Activity (Order SAF formulation)
Flow Cytometry: 0.5-1 μg/million cells in 0.1ml
Immunofluorescence: 1-2 μg/mL
Immunohistochemistry (FFPE): 0.5-1 μg/mL for 30 min at RT (1)

Restrictions: For Research Use only

Handling

Buffer: In 1X PBS, BSA free, sodium azide free

Preservative: Azide free

Storage: 4 °C,-20 °C

Storage Comment: 2-8°C. The azide-free format should be aliquoted and stored at -20°C or colder.

Target Details for TLR4

Target: TLR4 (Toll-Like Receptor 4 (TLR4))

Alternative Name: Toll-like receptor 4

Background:

Target Description: This mAb reacts with human Toll-like receptor 2 (TLR4). It is a member of the Toll-like receptor (TLR) family, which plays a fundamental role in pathogen recognition and activation of innate immunity. TLRs are highly conserved from Drosophila to humans and share structural and functional similarities. They recognize pathogen-associated molecular patterns that are expressed on infectious agents, and mediate the production of cytokines necessary for the development of effective immunity. The various TLRs exhibit different patterns of expression. This receptor has been implicated in signal transduction events induced by lipopolysaccharide (LPS) found in most gram-negative bacteria. Mutations in this gene have been associated with differences in LPS responsiveness. Multiple transcript variants encoding different isoforms have been found for this gene.

Gene Symbol: TLR4

Gene ID: 7099

UniProt: O00206

Pathways: TLR Signaling, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Toll-Like Receptors Cascades, Inflammasome, S100 Proteins