EpCAM antibody

Catalog No. ABIN6297644

The Mouse Monoclonal anti-EpCAM antibody has been validated for WB, FACS, IF and IHC. It is suitable to detect EpCAM in samples from Human.

Quick Overview for EpCAM antibody (ABIN6297644)

Target: EpCAM (EPCAM) (Epithelial Cell Adhesion Molecule (EPCAM))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This EpCAM antibody is un-conjugated

Application: Western Blotting (WB), Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (IHC)

Product Details anti-EpCAM Antibody

Purpose: Mouse anti-Human EpCAM Antibody [Sodium Azide Free]

Specificity: Cell surface

Purification: Small cell lung carcinoma cells were used as the immunogen for the anti-EpCAM antibody.

Immunogen: Small cell lung carcinoma cells were used as the immunogen for the anti-EpCAM antibody.

Application Details

Application Notes: Flow Cytometry: 0.5-1 μg/million cells in 0.1ml
Immunofluorescence: 1-2 μg/mL
Western blot: 0.5-1 μg/mL
Immunohistochemistry (FFPE): 0.5-1 μg/mL for 30 min at RT (1)
Prediluted format: incubate for 30 min at RT (2)

Restrictions: For Research Use only

Handling

Buffer: In 1X PBS, BSA free, sodium azide free

Preservative: Azide free

Storage: 4 °C,-20 °C

Storage Comment: 2-8°C. The azide-free format should be aliquoted and stored at -20°C or colder.

Target Details for EpCAM

Target: EpCAM (EPCAM) (Epithelial Cell Adhesion Molecule (EPCAM))

Alternative Name: Epithelial cell adhesion molecule

Background:

Target Description: EGP40 is a 40-43 kDa transmembrane epithelial glycoprotein, also identified as epithelial specific antigen (ESA), or epithelial cellular adhesion molecule (Ep-CAM). It is expressed on baso-lateral cell surface in most simple epithelia and a vast majority of carcinomas. This antibody has been used to distinguish adenocarcinoma from pleural mesothelioma and hepatocellular carcinoma. This antibody is also useful in distinguishing serous carcinomas of the ovary from mesothelioma.

Gene Symbol: EPCAM///GA733-2///M1S2///M4S1///MIC18///TACSTD1///TROP1

Gene ID: 4072

UniProt: P16422