Nuclear Antigen antibody

Catalog No. ABIN6297661

This Mouse Monoclonal antibody specifically detects Nuclear Antigen in FACS, IF and IHC. It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for Nuclear Antigen antibody (ABIN6297661)

Target: Nuclear Antigen (IPO-38)

Reactivity: Human, Mouse, Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: This Nuclear Antigen antibody is un-conjugated

Application: Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (IHC)

Product Details anti-Nuclear Antigen Antibody

Purpose: Mouse anti-Human/Mouse/Rat IPO38 Antibody (Proliferation Marker) [Sodium Azide Free]

Specificity: Nuclear

Purification: Spleen cells from a patient with hairy cell leukemia were used as the immunogen for the IPO38 antibody.

Immunogen: Spleen cells from a patient with hairy cell leukemia were used as the immunogen for the IPO38 antibody.

Application Details

Application Notes: Flow Cytometry: 0.5-1 μg/million cells in 0.1ml
Immunofluorescence: 0.5-1 μg/mL
Immunohistochemistry (FFPE): 0.5-1 μg/mL for 30 min at RT (1)
Prediluted format: incubate for 30 min at RT (2)

Restrictions: For Research Use only

Handling

Buffer: In 1X PBS, BSA free, sodium azide free

Preservative: Azide free

Storage: 4 °C,-20 °C

Storage Comment: 2-8°C. The azide-free format should be aliquoted and stored at -20°C or colder.

Target Details for Nuclear Antigen

Target: Nuclear Antigen (IPO-38)

Alternative Name: IPO38

Background:

Target Description: Recognizes a protein of 14-16 kDa, which is a novel nuclear antigen of proliferating cells. IPO-38 antigen is present in the nuclei of proliferating cells such as Hodgkin s disease and non-Hodgkin s lymphomas, different forms of leukemias, breast and colorectal carcinomas, and PHA-stimulated lymphocytes. It is not expressed in the cells of non-stimulated lymphocytes and granulocytes. IPO-38 can be a useful marker of cell proliferation during monitoring of tumor progression.