CD43 antibody

Catalog No. ABIN6297682

This Mouse Monoclonal antibody specifically detects CD43 in FACS, WB, IHC and IF. It exhibits reactivity toward Human.

Quick Overview for CD43 antibody (ABIN6297682)

Target: CD43 (SPN) (Sialophorin (SPN))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD43 antibody is un-conjugated

Application: Flow Cytometry (FACS), Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

Product Details anti-CD43 Antibody

Purpose: Mouse anti-Human CD43 Antibody [Sodium Azide Free]

Specificity: Cell surface

Purification: Stimulated human leukocytes were used as the immunogen for the CD43 antibody.

Immunogen: Stimulated human leukocytes were used as the immunogen for the CD43 antibody.

Application Details

Application Notes: Western blot: 1-2 μg/mL
Flow Cytometry: 0.5-1 μg/million cells in 0.1ml
Immunofluorescence: 1-2 μg/mL
Immunohistochemistry (FFPE): 0.5-1 μg/mL for 30 min at RT (1)
Prediluted format: incubate for 30 min at RT (2)

Restrictions: For Research Use only

Handling

Buffer: In 1X PBS, BSA free, sodium azide free

Preservative: Azide free

Storage: 4 °C,-20 °C

Storage Comment: 2-8°C. The azide-free format should be aliquoted and stored at -20°C or colder.

Target Details for CD43

Target: CD43 (SPN) (Sialophorin (SPN))

Alternative Name: Leukosialin

Background:

Target Description: It recognizes a cell surface glycoprotein of 95/115/135 kDa (depending upon the extent of glycosylation), identified as CD43 (Workshop III). 70-90 % of T-cell lymphomas and from 22-37 % of B-cell lymphomas express CD43. No reactivity has been observed with reactive B-cells. So a B-lineage population that co-expresses CD43 is highly likely to be a malignant lymphoma, especially a low-grade lymphoma, rather than a reactive B-cell population. When CD43 antibody is used in combination with anti-CD20, effective immunophenotyping of the lymphomas in formalin-fixed tissues can be obtained. Co-staining of a lymphoid infiltrate with anti-CD20 and anti-CD43 argues against a reactive process and favors a diagnosis of lymphoma.

Gene Symbol: SPN///CD43

Gene ID: 6693

UniProt: P16150

Pathways: Regulation of Leukocyte Mediated Immunity