Recombinant Golgi (full length) antibody

Catalog No. ABIN6654321

This Rabbit Monoclonal antibody specifically detects Golgi in IHC (p). It exhibits reactivity toward Human.

Quick Overview for Recombinant Golgi (full length) antibody (ABIN6654321)

Target: Golgi

Antibody Type: Recombinant Antibody

Reactivity: Human

Host: Rabbit

Clonality: Monoclonal

Conjugate: Un-conjugated

Application: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: GLG1-2829R

Product Details

Binding Specificity: full length

Purpose: Recombinant Golgi Antibody

Purification: Protein A affinity chromatography

Immunogen: Full length recombinant GLG1 protein was used as the immunogen for this recombinant Golgi antibody.

Isotype: IgG, kappa

Application Details

Application Notes: The optimal dilution of the recombinant Golgi antibody for each application should be determined by the researcher.

1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.2 mg/mL

Buffer: 0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store the recombinant Golgi antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Target Details

Target: Golgi

Background: This MAb recognizes an antigen associated with the Golgi complex in human cells only. It can be used to stain the Golgi complex in cell or tissue preparations and can be used as a Golgi marker in subcellular fractions. It produces a diffuse staining pattern of the Golgi zone in normal and malignant cells. This MAb is an excellent marker for human cells in xenographic model research. It reacts specifically with human cells. The Golgi apparatus is an organelle present in all eukaryotic cells that forms a part of the endomembrane system. The primary function of the Golgi apparatus is to process and package macromolecules synthesized by the cell for exocytosis or use within the cell. The Golgi is made up of a stack of flattened, membrane-bound sacs known as cisternae, with three functional regions: the cis face, medial region and trans face. Each region consists of various enzymes that selectively modify the macromolecules passing though them, depending on where they are destined to reside. Several spherical vesicles that have budded off of the Golgi are present surrounding the main cisternae.

UniProt: Q92896