Golgi antibody (CF®647)

Catalog No. ABIN7880913

The Mouse Monoclonal anti-Golgi antibody is suitable to detect Golgi in samples from Human. It has been validated for FACS and IF.

Quick Overview for Golgi antibody (CF®647) (ABIN7880913)

Target: Golgi

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: CF®647

Application: Flow Cytometry (FACS), Immunofluorescence (IF)

Clone: AE-6

Product Details

Purpose: Golgi Antibody

Purification: Protein G affinity chromatography

Immunogen: SU-DHL-1 large cell lymphoma cells were used as the immunogen for the Golgi antibody.

Isotype: IgG1, kappa

Application Details

Application Notes: Optimal dilution of the Golgi antibody should be determined by the researcher.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: 500 µL at 0.1 mg/mL with 0.1 mg/mL BSA (US sourced), 0.05 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Store the Golgi antibody at 2-8oC, protected from light.

Target Details

Target: Golgi

Background: This MAb recognizes an antigen associated with the Golgi complex in human cells only. It can be used to stain the Golgi complex in cell or tissue preparations and can be used as a Golgi marker in subcellular fractions. It produces a diffuse staining pattern of the Golgi zone in normal and malignant cells. This MAb is an excellent marker for human cells in xenographic model research. It reacts specifically with human cells. The Golgi apparatus is an organelle present in all eukaryotic cells that forms a part of the endomembrane system. The primary function of the Golgi apparatus is to process and package macromolecules synthesized by the cell for exocytosis or use within the cell. The Golgi is made up of a stack of flattened, membrane-bound sacs known as cisternae, with three functional regions: the cis face, medial region and trans face. Each region consists of various enzymes that selectively modify the macromolecules passing though them, depending on where they are destined to reside. Several spherical vesicles that have budded off of the Golgi are present surrounding the main cisternae. The Golgi tends to be more pronounced and numerous in cells that make and secrete many substances such as plasma B cells.