Melanoma gp100 antibody

Catalog No. ABIN6297733

This Mouse Monoclonal antibody specifically detects Melanoma gp100 in IHC, WB, IF and FACS. It exhibits reactivity toward Human.

Quick Overview for Melanoma gp100 antibody (ABIN6297733)

Target: Melanoma gp100 (PMEL) (Premelanosome Protein (PMEL))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This Melanoma gp100 antibody is un-conjugated

Application: Immunohistochemistry (IHC), Western Blotting (WB), Immunofluorescence (IF), Flow Cytometry (FACS)

Product Details anti-Melanoma gp100 Antibody

Purpose: Mouse anti-Human gp100 Antibody [Sodium Azide Free]

Specificity: Cytoplasmic

Purification: The extract of pigmented melanoma metastases from lymph nodes was used as the immunogen for this gp100 antibody.

Immunogen: The extract of pigmented melanoma metastases from lymph nodes was used as the immunogen for this gp100 antibody.

Application Details

Application Notes: Western Blot: 1-2 μg/mL
FACS:0.5-1 μg/million cells
IF: 0.5-1 μg/mL
IHC (FFPE): 0.5-1.0 μg/mL for 30 minutes at RT (1)
Prediluted format: incubate for 30 min at RT (2)

Restrictions: For Research Use only

Handling

Buffer: In 1X PBS ( pH 7.4), BSA free, sodium azide free

Preservative: Azide free

Storage: 4 °C,-20 °C

Storage Comment: 2-8°C. The azide-free format should be aliquoted and stored at -20°C or colder.

Target Details for Melanoma gp100

Target: Melanoma gp100 (PMEL) (Premelanosome Protein (PMEL))

Alternative Name: Melanocyte protein PMEL , M-beta]

Background:

Target Description: Melanocytes produce organelles called melanosomes which produce melanin, the pigment that gives color to skin, hair, eyes, scales and feathers. gp100 was identified in an attempt to clone the gene Tyrosinase, an enzyme required for melanin synthesis. Further testing determined that gp100 is a melanoma-specific protein and is responsible for melanosome maturation, facilitating the transition from amorphous rounded vesicles to fibrillary ellipsoid organelles.
Metastatic amelanotic melanoma can often be confused with a variety of poorly differentiated carcinomas, large cell lymphomas, and sarcomas using H & E stains alone. It is also difficult to differentiate melanoma from spindle cell carcinomas and various types of mesenchymal neoplasms. Clone HMB45 gp100 antibody stains fetal and neonatal melanocytes, junctional and blue nevus cells, and malignant melanoma. It also stains angiomyolipomas, tumors most commonly associated with the kidney. Intradermal nevi, normal adult melanocytes, and non-melanocytic cells are negative. This gp100 antibody does not stain tumor cells of epithelial, lymphoid, glial, or mesenchymal origin.

Gene Symbol: PMEL///D12S53E///PMEL17///SILV

Gene ID: 6490

UniProt: P40967