Insulin antibody
Quick Overview for Insulin antibody (ABIN6386407)
Target
See all Insulin (INS) AntibodiesReactivity
Host
Clonality
Conjugate
Application
Clone
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Specificity
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Insulin, human
MAbs 7F8, D4B8cc, RC3A6 and RC8E2 react with different antigenic determinants. -
Cross-Reactivity
- Cow (Bovine), Pig (Porcine)
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Cross-Reactivity (Details)
- These antibodies cross-react with human proinsulin, bovine insulin (30 % ) and porcine insulin. No cross-reaction with free C-peptide.
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Purification
- Chromatography on protein A Sepharose
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Immunogen
- Mouse monoclonal antibody produced in bioreactor. Hybridoma clone derived from hybridization of X63-Ag8-653 myeloma cells with spleen cells of Balb/c mice.
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Isotype
- IgG1 kappa
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Application Notes
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Detection of insulin in two-site enzyme immunoassay. Following pairs are recommended (capture-detection):
7F8 - D4B8cc, RC3A6 - RC8E2.
MAb D4B8cc can be used in immunohistochemistry on frozen sections. -
Restrictions
- For Research Use only
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Buffer
- PBS, pH 7.4, 0.09 % sodium azide (NaN3)
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Preservative
- Sodium azide
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Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Storage
- 4 °C
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- Insulin (INS)
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Alternative Name
- Insulin
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Pathways
- NF-kappaB Signaling, RTK Signaling, Positive Regulation of Peptide Hormone Secretion, Peptide Hormone Metabolism, Hormone Activity, Carbohydrate Homeostasis, ER-Nucleus Signaling, Regulation of Carbohydrate Metabolic Process, Feeding Behaviour, Autophagy, Negative Regulation of intrinsic apoptotic Signaling, Brown Fat Cell Differentiation, Positive Regulation of fat Cell Differentiation
Target
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