AKT1 antibody (pThr308)

Catalog No. ABIN650880

This anti-AKT1 antibody is a Rabbit Polyclonal antibody detecting AKT1 in IF and DB. Suitable for Human. This Primary Antibody has been cited in 3+ publications.

Quick Overview for AKT1 antibody (pThr308) (ABIN650880)

Target: AKT1 (V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This AKT1 antibody is un-conjugated

Application: Immunofluorescence (IF), Dot Blot (DB)

Clone: RB27685

Product Details anti-AKT1 Antibody

Binding Specificity: pThr308

Purification: This antibody is purified through a protein A column, followed by peptide affinity purification.

Immunogen: This AKT1 Antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding Thr308 of human AKT1.

Isotype: Ig Fraction

Application Details

Application Notes: IF: 1:10~50. DB: 1:500

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Purified polyclonal antibody supplied in PBS with 0.09 % (W/V) sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots to prevent freeze-thaw cycles.

Expiry Date: 6 months

References for anti-AKT1 antibody (ABIN650880)

Li, Miao, Shan, Liu, Li, Zhao, Jia: "MiR-106b and miR-93 regulate cell progression by suppression of PTEN via PI3K/Akt pathway in breast cancer." in: Cell death & disease, Vol. 8, Issue 5, pp. e2796, (2018) (PubMed).

Zhao, Shan, Liu, Li, Jia: "Functional screen analysis reveals miR-3142 as central regulator in chemoresistance and proliferation through activation of the PTEN-AKT pathway in CML." in: Cell death & disease, Vol. 8, Issue 5, pp. e2830, (2018) (PubMed).

Miao, Zheng, Li, Su, Zhao, Zhou, Jia: "MicroRNA-130b targets PTEN to mediate drug resistance and proliferation of breast cancer cells via the PI3K/Akt signaling pathway." in: Scientific reports, Vol. 7, pp. 41942, (2017) (PubMed).

Target Details for AKT1

Target: AKT1 (V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1))

Alternative Name: AKT1

Background: The serine-threonine protein kinase encoded by the AKT1 gene is catalytically inactive in serum-starved primary and immortalized fibroblasts. AKT1 and the related AKT2 are activated by platelet-derived growth factor. The activation is rapid and specific, and it is abrogated by mutations in the pleckstrin homology domain of AKT1. It was shown that the activation occurs through phosphatidylinositol 3-kinase. In the developing nervous system AKT is a critical mediator of growth factor-induced neuronal survival. Survival factors can suppress apoptosis in a transcription-independent manner by activating the serine/threonine kinase AKT1, which then phosphorylates and inactivates components of the apoptotic machinery. Multiple alternatively spliced transcript variants have been found for this gene. [provided by RefSeq].

Molecular Weight: 55686

Gene ID: 207

NCBI Accession: NP_001014431, NP_001014432, NP_005154

UniProt: P31749

Pathways: PI3K-Akt Signaling, RTK Signaling, TCR Signaling, AMPK Signaling, Interferon-gamma Pathway, TLR Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Response to Water Deprivation, Regulation of Actin Filament Polymerization, Carbohydrate Homeostasis, Glycosaminoglycan Metabolic Process, Cellular Glucan Metabolic Process, Regulation of Muscle Cell Differentiation, Cell-Cell Junction Organization, Regulation of Cell Size, Skeletal Muscle Fiber Development, Regulation of Carbohydrate Metabolic Process, Hepatitis C, Protein targeting to Nucleus, CXCR4-mediated Signaling Events, Signaling Events mediated by VEGFR1 and VEGFR2, Negative Regulation of intrinsic apoptotic Signaling, Thromboxane A2 Receptor Signaling, Signaling of Hepatocyte Growth Factor Receptor, Positive Regulation of fat Cell Differentiation, VEGFR1 Specific Signals, VEGF Signaling, Warburg Effect