FOXO1 antibody (AA 242-655)

Catalog No. ABIN189997

This Mouse Monoclonal antibody specifically detects FOXO1 in WB, IHC (p) and IF/ICC. It exhibits reactivity toward Human.

Quick Overview for FOXO1 antibody (AA 242-655) (ABIN189997)

Target: FOXO1 (Forkhead Box O1 (FOXO1))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This FOXO1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (fixed cells) (IF/ICC)

Clone: 7H3

Product Details anti-FOXO1 Antibody

Binding Specificity: AA 242-655

Purpose: Anti-FKHR Mouse Monoclonal Antibody

Specificity: This antibody recognizes human FKHR/FOX01.

Cross-Reactivity: Human

Immunogen: GST fusion protein containing the region encoding aa 242-655 of human FKHR expressed in E. coli.

Isotype: IgG1

Application Details

Application Notes: Immunoblotting: use at 0.1-2.0 μg/mL. A band of 70 kDa is detected.
Endusers should determine optimal concentrations for their applications.

Restrictions: For Research Use only

Handling

Format: Liquid

Reconstitution: Dilute in PBS or medium that is identical to that used in the assay system.

Concentration: Lot specific

Buffer: PBS, pH 7.4

Storage: -20 °C

Storage Comment: This antibody is stable for at least one (1) year at -20°C or below. Store undiluted product in appropriate aliquots to avoid multiple freeze-thaw cycles.

Target Details for FOXO1

Target: FOXO1 (Forkhead Box O1 (FOXO1))

Alternative Name: FOXO1A

Background: Forkhead box protein O1,Transcription factor that is the main target of insulin signaling and regulates metabolic homeostasis in response to oxidative stress (PubMed:10358076, PubMed:12228231, PubMed:15220471, PubMed:15890677, PubMed:18356527, PubMed:19221179, PubMed:20543840, PubMed:21245099). Binds to the insulin response element (IRE) with consensus sequence 5'-TT[G/A]TTTTG-3' and the related Daf-16 family binding element (DBE) with consensus sequence 5'-TT[G/A]TTTAC-3' (PubMed:10358076). Activity suppressed by insulin (PubMed:10358076). Main regulator of redox balance and osteoblast numbers and controls bone mass (By similarity). Orchestrates the endocrine function of the skeleton in regulating glucose metabolism (By similarity). Also acts as a key regulator of chondrogenic commitment of skeletal progenitor cells in response to lipid availability: when lipids levels are low, translocates to the nucleus and promotes expression of SOX9, which induces chondrogenic commitment and suppresses fatty acid oxidation (By similarity). Acts synergistically with ATF4 to suppress osteocalcin/BGLAP activity, increasing glucose levels and triggering glucose intolerance and insulin insensitivity (By similarity). Also suppresses the transcriptional activity of RUNX2, an upstream activator of osteocalcin/BGLAP (By similarity). In hepatocytes, promotes gluconeogenesis by acting together with PPARGC1A and CEBPA to activate the expression of genes such as IGFBP1, G6PC1 and PCK1 (By similarity). Important regulator of cell death acting downstream of CDK1, PKB/AKT1 and STK4/MST1 (PubMed:18356527, PubMed:19221179). Promotes neural cell death (PubMed:18356527). Mediates insulin action on adipose tissue (By similarity). Regulates the expression of adipogenic genes such as PPARG during preadipocyte differentiation and, adipocyte size and adipose tissue-specific gene expression in response to excessive calorie intake (By similarity). Regulates the transcriptional activity of GADD45A and repair of nitric oxide-damaged DNA in beta-cells (By similarity). Required for the autophagic cell death induction in response to starvation or oxidative stress in a transcription-independent manner (PubMed:20543840). Mediates the function of MLIP in cardiomyocytes hypertrophy and cardiac remodeling (By similarity). Regulates endothelial cell (EC) viability and apoptosis in a PPIA/CYPA-dependent manner via transcription of CCL2 and BCL2L11 which are involved in EC chemotaxis and apoptosis (PubMed:31063815). {UniProtKB:A4L7N3, UniProtKB:G3V7R4, UniProtKB:Q9R1E0, PubMed:10358076, PubMed:12228231, PubMed:15220471, PubMed:15890677, PubMed:18356527, PubMed:19221179, PubMed:20543840, PubMed:21245099, PubMed:31063815}.,FKHR (ForKHead in Rhabdomyosarcoma), a 70 kDa protein, is a member of the forkhead family of transcription factors. Members of this family are classified by their degree of homology in the 100-120aa region that is necessary for DNA binding and binding-site specificity. The FKHR subfamily differs from other members of the forkhead family in that all members contain an insertion of 5 amino acids next to the helix 3 in the middle of the forkhead domain and lack the conserved amino terminal KPPY sequence found in most forkhead genes. It has been shown that members of the FKHR gene family are phosphorylated by Akt which regulates its cellular localization promoting cell survival.,Cytoplasm, Nucleus,Forkhead box protein O1A, Forkhead in rhabdomyosarcoma

Gene ID: 2308

NCBI Accession: NP_002006

UniProt: Q12778

Pathways: PI3K-Akt Signaling, Cell Division Cycle, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Carbohydrate Homeostasis, Chromatin Binding, Regulation of Carbohydrate Metabolic Process, CXCR4-mediated Signaling Events, BCR Signaling