RFC4 antibody

Catalog No. ABIN190320

The Mouse Monoclonal anti-RFC4 antibody (Clone 1320) (ABIN190320) specifically detects RFC4 in WB and IF/ICC. The antibody is reactive with Human samples.

Quick Overview for RFC4 antibody (ABIN190320)

Target: RFC4 (Replication Factor C (Activator 1) 4, 37kDa (RFC4))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This RFC4 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (fixed cells) (IF/ICC)

Clone: 1320

Product Details anti-RFC4 Antibody

Purpose: Anti-Replication Factor C p37 Mouse Monoclonal Antibody

Specificity: This antibody recognizes the p37 subunit of human and yeast (S. cerevisiae) Replication Factor C (RFC). RFC is a heteropentameric protein consisting of p140, p40, p38, p37, and p36 subunits, RFC participates in targeting DNA polymerase delta processivity factor (PCNA) on to a primed DNA template during DNA synthesis and repair. RFC has also been associated with DNA repair in conjunction with a PCNA-like heterotrimer of Hus1, Rad1, and Rad9.

Cross-Reactivity: Human, Saccharomyces cerevisiae

Immunogen: Recombinant protein corresponding to the p37 subunit of human Replication Factor C expressed in E. coli.

Isotype: IgG2b

Application Details

Application Notes: Immunoblotting: use at 1:2,000 dilution. A band of 37 kD is detected.
Positive controls: HeLa cell lysate.

Restrictions: For Research Use only

Handling

Format: Liquid

Reconstitution: Dilute in PBS or medium which is identical to that used in the assay system.

Concentration: Lot specific

Buffer: PBS, pH 7.4

Storage: -80 °C

Storage Comment: This antibody is stable for at least one (1) year at -70°C. Avoid multiple freeze-thaw cycles.

Target Details for RFC4

Target: RFC4 (Replication Factor C (Activator 1) 4, 37kDa (RFC4))

Alternative Name: RFC4 / RFC37

Background: Replication factor C subunit 2,The elongation of primed DNA templates by DNA polymerase delta and epsilon requires the action of the accessory proteins proliferating cell nuclear antigen (PCNA) and activator 1. This subunit binds ATP (By similarity). {ECO:0000250}.,Nucleus,Activator 1 40 kDa subunit, A1 40 kDa subunit, Activator 1 subunit 2, Replication factor C 40 kDa subunit, RF-C 40 kDa subunit, RFC40

UniProt: P35250

Pathways: Telomere Maintenance, DNA Damage Repair, DNA Replication, Synthesis of DNA