PCK1 antibody (AA 491-540)

Catalog No. ABIN6579424

The Rabbit Polyclonal anti-PCK1 antibody is suitable to detect PCK1 in samples from Human, Mouse and Rat. It has been validated for WB, ELISA, IHC and IF.

Quick Overview for PCK1 antibody (AA 491-540) (ABIN6579424)

Target: PCK1 (phosphoenolpyruvate Carboxykinase 1 (Soluble) (PCK1))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PCK1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF)

Product Details anti-PCK1 Antibody

Binding Specificity: AA 491-540

Purpose: PEPCK-C Antibody

Specificity: PEPCK-C Polyclonal Antibody detects endogenous levels of PEPCK-C protein.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunogen: The antiserum was produced against synthesized peptide derived from the Internal region of human PCK1. AA range:491-540

Isotype: IgG

Application Details

Application Notes: WB 1:500 - 1:2000. IHC: 1:100-300 ELISA: 1:20000.. IF 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C/1 year

Expiry Date: 12 months

Target Details for PCK1

Target: PCK1 (phosphoenolpyruvate Carboxykinase 1 (Soluble) (PCK1))

Alternative Name: PCK1

Background:

Synonyms: PCK1, PEPCK1, Phosphoenolpyruvate carboxykinase, cytosolic [GTP], PEPCK-C, Phosphoenolpyruvate carboxylase

Background: This gene is a main control point for the regulation of gluconeogenesis. The cytosolic enzyme encoded by this gene, along with GTP, catalyzes the formation of phosphoenolpyruvate from oxaloacetate, with the release of carbon dioxide and GDP. The expression of this gene can be regulated by insulin, glucocorticoids, glucagon, cAMP, and diet. Defects in this gene are a cause of cytosolic phosphoenolpyruvate carboxykinase deficiency. A mitochondrial isozyme of the encoded protein also has been characterized. [provided by RefSeq, Jul 2008],

Gene Name: PCK1

Protein Name: Phosphoenolpyruvate carboxykinase cytosolic [GTP]

Molecular Weight: 69 kDa

Gene ID: 5105

UniProt: P35558

Pathways: Positive Regulation of Peptide Hormone Secretion, Carbohydrate Homeostasis