H2AFX antibody (pSer139)

Catalog No. ABIN6654164

This anti-H2AFX antibody is a Rabbit Monoclonal antibody detecting H2AFX in WB, IF, IHC (p) and ICC. Suitable for Human and Mouse.

Quick Overview for H2AFX antibody (pSer139) (ABIN6654164)

Target: H2AFX (H2A Histone Family, Member X (H2AFX))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Monoclonal

Conjugate: This H2AFX antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunocytochemistry (ICC)

Clone: AbH41

Product Details anti-H2AFX Antibody

Binding Specificity: pSer139

Purification: Protein A affinity

Immunogen: The amino acids surrounding a phosphorylated serine at position 139 were used as the immunogen for the phospho-Histone H2AX antibody.

Isotype: IgG

Application Details

Application Notes: Optimal dilution of the phospho-Histone H2AX antibody should be determined by the researcher.\. Western blot: 0.5-1 μg/mL,IHC (FFPE): 1-2 μg/mL,IF/ICC: 2-4 μg/mL

Restrictions: For Research Use only

Handling

Buffer: Antibody in PBS with 0.02 % sodium azide and 50 % glycerol

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: After reconstitution, the phospho-Histone H2AX antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Target Details for H2AFX

Target: H2AFX (H2A Histone Family, Member X (H2AFX))

Alternative Name: Histone H2AX

Background: Variant histone H2A which replaces conventional H2A in a subset of nucleosomes. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. Required for checkpoint-mediated arrest of cell cycle progression in response to low doses of ionizing radiation and for efficient repair of DNA double strand breaks (DSBs) specifically when modified by C- terminal phosphorylation. [UniProt]

UniProt: P16104

Pathways: Telomere Maintenance, DNA Damage Repair, Positive Regulation of Response to DNA Damage Stimulus