H2AFX antibody (pSer139)

Catalog No. ABIN6654164

The Rabbit Monoclonal anti-H2AFX antibody (Clone AbH41) (ABIN6654164) specifically detects H2AFX in WB, IF, ICC and IHC (p). The antibody is reactive with Human and Mouse samples.

Quick Overview for H2AFX antibody (pSer139) (ABIN6654164)

Target: H2AFX (H2A Histone Family, Member X (H2AFX))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Monoclonal

Conjugate: This H2AFX antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunocytochemistry (ICC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: AbH41

Product Details anti-H2AFX Antibody

Binding Specificity: pSer139

Purification: Protein A affinity

Immunogen: The amino acids surrounding a phosphorylated serine at position 139 were used as the immunogen for the phospho-Histone H2AX antibody.

Isotype: IgG

Application Details

Application Notes: Optimal dilution of the phospho-Histone H2AX antibody should be determined by the researcher.\. Western blot: 0.5-1 μg/mL,IHC (FFPE): 1-2 μg/mL,IF/ICC: 2-4 μg/mL

Restrictions: For Research Use only

Handling

Buffer: Antibody in PBS with 0.02 % sodium azide and 50 % glycerol

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: After reconstitution, the phospho-Histone H2AX antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Target Details for H2AFX

Target: H2AFX (H2A Histone Family, Member X (H2AFX))

Alternative Name: Histone H2AX

Background: Variant histone H2A which replaces conventional H2A in a subset of nucleosomes. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. Required for checkpoint-mediated arrest of cell cycle progression in response to low doses of ionizing radiation and for efficient repair of DNA double strand breaks (DSBs) specifically when modified by C- terminal phosphorylation. [UniProt]

UniProt: P16104

Pathways: Telomere Maintenance, DNA Damage Repair, Positive Regulation of Response to DNA Damage Stimulus