APEX1 antibody (full length)

Catalog No. ABIN6654473

The Mouse Monoclonal anti-APEX1 antibody (Clone CPTC-APEX1-2) (ABIN6654473) specifically detects APEX1 in WB, IF, IHC (p) and FACS. The antibody is reactive with Human samples.

Quick Overview for APEX1 antibody (full length) (ABIN6654473)

Target: APEX1 (Apurinic/Apyrimidinic Endonuclease 1 (APEX1))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This APEX1 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Flow Cytometry (FACS)

Clone: CPTC-APEX1-2

Product Details anti-APEX1 Antibody

Binding Specificity: full length

Purpose: APE1 Antibody / APEX1

Purification: Protein G affinity chromatography

Immunogen: Recombinant full-length human protein was used as the immunogen for the APE1 antibody.

Isotype: IgG1, kappa

Application Details

Application Notes: Optimal dilution of the APE1 antibody to be determined by the researcher.

1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.2 mg/mL

Buffer: 0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store the APE1 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Target Details for APEX1

Target: APEX1 (Apurinic/Apyrimidinic Endonuclease 1 (APEX1))

Alternative Name: APE1

Background: APEX / APE1 is a multifunctional protein that plays a central role in the cellular response to oxidative stress. The two major activities of APEX1 in DNA repair and redox regulation of transcriptional factors. Functions as a apurinic/apyrimidinic (AP) endodeoxyribonuclease in the DNA base excision repair (BER) pathway of DNA lesions induced by oxidative and alkylating agents. Patients with genetic variants in APEX1 and XRCC1 have been shown to have a higher risk of lung cancer. Elevated APEX1 levels observed in human testicular cancer may be related to relative resistance to therapy and therefore may serve as a diagnostic marker for refractory disease.

UniProt: P27695

Pathways: DNA Damage Repair, Chromatin Binding, Cell RedoxHomeostasis, Smooth Muscle Cell Migration, Positive Regulation of Response to DNA Damage Stimulus