Recombinant MUC1 antibody

Catalog No. ABIN6654782

The Mouse Monoclonal anti-MUC1 antibody (Clone rMUC1-960) (ABIN6654782) specifically detects MUC1 in WB and IHC (p). The antibody is reactive with Human samples.

Quick Overview for Recombinant MUC1 antibody (ABIN6654782)

Target: MUC1 (Mucin 1 (MUC1))

Antibody Type: Recombinant Antibody

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This MUC1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: RMUC1-960

Product Details anti-MUC1 Antibody

Purpose: Recombinant MUC-1 Antibody

Purification: Protein G affinity chromatography

Immunogen: Human milk fat globule membranes were used as the immunogen for this recombinant MUC-1 antibody.

Isotype: IgG2b, kappa

Application Details

Application Notes: The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the recombinant MUC-1 antibody to be titered up or down for optimal performance.

1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.2 mg/mL

Buffer: 0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store the recombinant MUC-1 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Target Details for MUC1

Target: MUC1 (Mucin 1 (MUC1))

Alternative Name: MUC-1

Background: This mAb reacts with MUC1, a large transmembrane glycoprotein expressed on the ductal surface of normal glandular epithelia. The dominant epitope of this MAb involves both amino acids as well as sugar moieties. Neuraminidase treatment destroys the antigen. It is a very good tracer agent in CA15.3 assays. The extracellular domain of MUC1 largely consists of a highly conserved, O-glycosylated 20 amino acids tandem repeat which can occur 30-100 times per molecule depending on the length of the allele involved. In the vast majority of human carcinomas this protein is up-regulated and poorly glycosylated and appears on the cell surface in a non-polarized fashion.

UniProt: P15941

Pathways: Negative Regulation of intrinsic apoptotic Signaling