Androgen Receptor antibody (N-Term, Ser213)

Catalog No. ABIN6655076

Supplier Product No.: 200-301-h54

This anti-Androgen Receptor antibody is a Mouse Monoclonal antibody detecting Androgen Receptor in WB and IHC. Suitable for Human.

Quick Overview for Androgen Receptor antibody (N-Term, Ser213) (ABIN6655076)

Target: Androgen Receptor (AR)

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This Androgen Receptor antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Clone: 156C135-2

Product Details anti-Androgen Receptor Antibody

Binding Specificity: N-Term, Ser213

Supplier Product No.: 200-301-h54

Supplier: Rockland

Purpose: Androgen Receptor Antibody

Cross-Reactivity (Details): A BLAST analysis was used to suggest cross-reactivity with Anti-Androgen Receptor from human and New World Monkey on 100 % homology with the immunizing sequence.

Purification: Anti-Androgen Receptor Antibody was purified by Protein G chromatography.

Immunogen: Androgen Receptor Antibody was produced in mice by repeated immunizations with a synthetic peptide corresponding to amino acids near the n-terminus of the Androgen Receptor containing the serine 213 phosphorylated serine in the peptide sequence.

Isotype: IgG1 kappa

Application Details

Application Notes:

Immunohistochemistry_Dilution: User Optimized

Western_Blot_Dilution: 1-4 μg/mL

Comment:

Anti-Androgen Receptor Antibody is tested for use in IHC (p) and WB. Expect a band approximately 110kDa on specific lysates. Specific conditions for reactivity should be optimized by the end user.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 0.05 % BSA

Preservative: 0.05 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Expiry Date: 12 months

Target Details for Androgen Receptor

Target: Androgen Receptor (AR)

Alternative Name: Androgen Receptor

Background:

Synonyms: DHTR, NR3C4

Background: Anti-Androgen Receptor Antibody detects human Androgen receptor. The androgen receptor (AR) is an ~110 kDa androgen-dependent transcription factor that is a member of the steroid/nuclear receptor gene superfamily. The AR signaling pathway plays a key role in development and function of male reproductive organs, including the prostate and epididymis. AR also plays a role in nonreproductive organs, such as muscle, hair follicles, and brain. Abnormalities in the AR signaling pathway have been linked to a number of diseases, including prostate cancer, Kennedy's disease and male infertility. The PI3K/Akt signaling pathway plays an important role in regulating AR activity through phosphorylation of AR at Ser213/210 and Ser791/790. Growth factors or cytokines may induce phosphorylation of AR through the PI3K/Akt pathway. IGF-1 activates the phosphatidylinositol 3-kinase(PI3K)/AKT pathway in LNCap at high passage number and increases phosphorylation of of AR at Ser213/210 (see western blot) and Ser791/790. The western blot results also show that inhibition of the PI3K/Akt pathway by LY294002 prior to incubation with IGF-1 suppressed AR phosphorylation at Ser213/210. Activation of the PI3K/AKt pathway is thought to have a survival role in prostate cancer by protecting cells from apoptosis. Anti-Androgen Receptor Antibody is ideal for investigators involved in neuroscience, cytokines and growth factors, and transcription factors.

Gene Name: AR

Gene ID: 367

NCBI Accession: NP_000035

UniProt: P10275

Pathways: Nuclear Receptor Transcription Pathway, Intracellular Steroid Hormone Receptor Signaling Pathway, Steroid Hormone Mediated Signaling Pathway, Regulation of Intracellular Steroid Hormone Receptor Signaling, Nuclear Hormone Receptor Binding, Chromatin Binding