Androgen Receptor antibody (pSer94)

Catalog No. ABIN6655077

Supplier Product No.: 600-401-w93

This Rabbit Polyclonal antibody specifically detects Androgen Receptor in WB and ELISA. It exhibits reactivity toward Human.

Quick Overview for Androgen Receptor antibody (pSer94) (ABIN6655077)

Target: Androgen Receptor (AR)

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Androgen Receptor antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-Androgen Receptor Antibody

Binding Specificity: pSer94

Supplier Product No.: 600-401-w93

Supplier: Rockland

Purpose: Androgen Receptor phospho S94 Antibody

Cross-Reactivity (Details): This antibody is specific for phosphorylated Androgen Receptor.

Purification: Anti-Phospho-Ser94 Androgen Receptor Antibody was prepared from monospecific antiserum by immunoaffinity chromatography using phospho peptide coupled to agarose beads followed by solid phase adsorption(s) against non-phospho peptide and non-specific peptide to remove any unwanted reactivities.

Sterility: Sterile filtered

Immunogen: Anti-Phospho-Ser94 Androgen Receptor Antibody was produced in rabbits by repeated immunizations with a synthetic phospho-peptide corresponding to amino acid residues surrounding the phosphorylated Ser94 of human AR conjugated to KLH.

Isotype: IgG

Application Details

Application Notes:

ELISA_Dilution: 1:10,000

Western_Blot_Dilution: 1:1000

Comment:

Anti-Phospho-Ser94 Androgen Receptor Antibody is tested for Western blots. Expect a band at approximately ~110 kDa on specific lysates or tissues. Specific conditions for reactivity should be optimized by the end user.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer:

Buffer: 0.01 M HEPES, 0.15 M Sodium Chloride, pH 7.5

Stabilizer: 0.1 mg/mL Bovine Serum Albumin (BSA) - IgG and Protease free, 50 % (v/v) Glycerol

Preservative: None

Preservative: Without preservative

Storage: -20 °C

Storage Comment: Store vial at -20° C prior to opening in undiluted aliquots. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. Dilute only prior to immediate use.

Expiry Date: 12 months

Target Details for Androgen Receptor

Target: Androgen Receptor (AR)

Alternative Name: Androgen Receptor

Background:

Synonyms: Dihydrotestosterone receptor, Nuclear receptor subfamily 3 group C member 4

Background: The androgen receptor (AR) is a DNA-binding transcription factor that regulates genes critical for the development and maintenance of the male sexual phenotype. Defects in androgen receptor have been shown to play a role in prostate cancer, and inhibition of AR activity through modulation of signal transduction pathways may delay prostate cancer progression. Multiple phosphorylation sites have been identified on the androgen receptor that affect cross-talk between growth factor signaling and androgen in prostate development and cancer. One of these sites, at Ser94, appears constitutively phosphorylated and exhibits no response to treatments with stimulating hormone. The site at Ser94 is unique among the AR phosphorylation sites in that it does not achieve a maximal level of phosphorylation between translation and the initial round of nuclear import, having a strong bias for androgen-independent phosphorylation in the cytoplasm. Androgen Receptor pS94 Antibody is ideal for researchers interested in cancer research.

Gene Name: AR

Gene ID: 367

NCBI Accession: NP_001011645

UniProt: P10275

Pathways: Nuclear Receptor Transcription Pathway, Intracellular Steroid Hormone Receptor Signaling Pathway, Steroid Hormone Mediated Signaling Pathway, Regulation of Intracellular Steroid Hormone Receptor Signaling, Nuclear Hormone Receptor Binding, Chromatin Binding