Histone H2B antibody (C-Term)

Catalog No. ABIN6655251

Supplier Product No.: 600-401-x47

This Rabbit Polyclonal antibody specifically detects Histone H2B in WB, ELISA, IF, ChIP and FM. It exhibits reactivity toward Human.

Quick Overview for Histone H2B antibody (C-Term) (ABIN6655251)

Target: Histone H2B

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Histone H2B antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF), Chromatin Immunoprecipitation (ChIP), Fluorescence Microscopy (FM)

Product Details anti-Histone H2B Antibody

Binding Specificity: C-Term

Supplier Product No.: 600-401-x47

Supplier: Rockland

Purpose: Histone H2B pan Antibody

Cross-Reactivity (Details): Cross reactivity with other species was not tested.

Purification: Anti-Histone H2B pan Antibody was purified by affinity purification.

Sterility: Sterile filtered

Immunogen: Anti-Histone H2B pan Antibody was produced in rabbits by repeated immunizations with a synthetic peptide containing a sequence from the C-terminus of histone H4.

Isotype: IgG

Application Details

Application Notes:

ChIP_Dilution: 2 μg per IP

ELISA_Dilution: 1:1000 - 1:5000

IF_Microscopy_Dilution: 1:1,000

Western_Blot_Dilution: 1:2,000 - 1:10,000

Other: Dot Blot: 1:20,000

Comment:

Anti-Histone H2B pan Antibody has been tested by Chromatin Immunoprecipitation, western blot, Immunofluorescence, and ELISA. Specific conditions for reactivity should be optimized by the end user.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer:

Buffer: 0.01 M Sodium Phosphate, 0.25 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.05 % (w/v) Sodium Azide and 0.05 % ProClin 300

Preservative: ProClin, Sodium azide

Precaution of Use: This product contains Sodium azide and ProClin: POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Expiry Date: 12 months

Target Details for Histone H2B

Target: Histone H2B

Background:

Synonyms: Histone H2B type 1-C/E/F/G/I, Histone H2B.1A, Histone H2B.a, Histone H2B.g, Histone H2B.h, Histone H2B.k, Histone H2B.l

Background: Histones are the main constituents of the protein part of chromosomes of eukaryotic cells. They are rich in the amino acids arginine and lysine and have been greatly conserved during evolution. Histones pack the DNA into tight masses of chromatin. Two core histones of each class H2A, H2B, H3 and H4 assemble and are wrapped by 146 base pairs of DNA to form one octameric nucleosome. Histones play a internal role in the regulation of transcription, DNA repair, DNA replication and chromosomal stability. These different functions are established via a complex set of post-translational modifications which either directly or indirectly alter chromatin structure and DNA accessibility to facilitate transcriptional activation or repression or other nuclear processes. Anti-Histone H2B pan Antibody is ideal for research in Chromatin Remodeling, Gene Expression and Epigenetics.

Gene Name: HIST1H2BC

Gene ID: 255626

NCBI Accession: NP_733759

UniProt: Q96A08