CD11b antibody (FITC)

Catalog No. ABIN6655310

Supplier Product No.: 200-302-n20

This anti-CD11b antibody is a Mouse Monoclonal antibody detecting CD11b in FACS, IP and FM. Suitable for Human. This Primary Antibody has been cited in 2+ publications.

Quick Overview for CD11b antibody (FITC) (ABIN6655310)

Target: CD11b (ITGAM) (Integrin alpha M (ITGAM))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD11b antibody is conjugated to FITC

Application: Flow Cytometry (FACS), Immunoprecipitation (IP), Fluorescence Microscopy (FM)

Clone: ICRF44

Product Details anti-CD11b Antibody (FITC)

Supplier Product No.: 200-302-n20

Supplier: Rockland

Purpose: CD11b Fluorescein Antibody

Cross-Reactivity (Details): Reactivity is observed against human CD11b, Chimpanzee, Baboon, Cynomolgus, Rhesus, Common Marmoset, and Swine.

Purification: Fluorescein conjugated CD11b Monoclonal Antibody was purified from tissue culture supernatant via affinity chromatography and is directed against human CD11b.

Sterility: Sterile filtered

Immunogen: Anti-CD11b Antibody (Monoclonal) was produced by repeated immunizations with CD11b antigen.

Isotype: IgG1 kappa

Labeling Ratio: 4-6

Application Details

Application Notes:

Immunoprecipitation_Dilution: User Optimized

Flow_Cytometry_Dilution: 5 µL/1x10e6 cells or 100μL of whole blood

IF_Microscopy_Dilution: User Optimized

Comment:

Anti-CD11b has been tested in Flow Cytometry and is useful for Immunofluorescence and Immunoprecipitation. Researchers should determine optimal titers for applications that are not stated.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 0.2 % BSA (w/v)

Preservative: 0.09 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Store vial at 4° C prior to opening.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use.  DO NOT FREEZE. This product is light sensitive.

Expiry Date: 6 months

References for anti-CD11b antibody (FITC) (ABIN6655310)

Guo, Wang, Dong, Lv, Zhao, Jin, Guo, Wang, Cai, Sun, Zhou, Zhang, Chen, Yang, Ji, Xin, Li, Li, Fan: "Effects of Ginkgo biloba on Early Decompression after Spinal Cord Injury." in: Evidence-based complementary and alternative medicine : eCAM, Vol. 2020, pp. 6958246, (2020) (PubMed).

Stewart, Thiel, Hogg: "Leukocyte integrins." in: Current opinion in cell biology, Vol. 7, Issue 5, pp. 690-6, (1996) (PubMed).

Target Details for CD11b

Target: CD11b (ITGAM) (Integrin alpha M (ITGAM))

Alternative Name: CD11b

Background:

Synonyms: Integrin alpha-M, CD11 antigen-like family member B, CR-3 alpha chain, Cell surface glycoprotein MAC-1 subunit alpha, Leukocyte adhesion receptor MO1, Neutrophil adherence receptor, CD11b, CR3A

Background: CD11b is a 165-170 kD type I transmembrane glycoprotein also known as αM integrin, Mac-1, CR3, and C3biR. CD11b non-covalently associates with integrin β2 (CD18) and is expressed on granulocytes, monocytes/macrophages, dendritic cells, NK cells, and subsets of T and B cells. CD11b/CD18 is critical for the transendothelial migration of monocytes and neutrophils. It is also involved in granulocyte adhesion, phagocytosis, and neutrophil activation. CD11b/CD18 interacts with ICAM-1 (CD54), ICAM-2 (CD102), ICAM-4, CD14, CD23, heparin, iC3b, fibrinogen, and Factor X.

Gene Name: ITGAM

Gene ID: 3684

NCBI Accession: NP_000623

UniProt: P11215

Pathways: Apoptosis, Activation of Innate immune Response, Toll-Like Receptors Cascades, Activated T Cell Proliferation