CD11b antibody (PE)

Catalog No. ABIN6655311

Supplier Product No.: 200-308-n20

This Mouse Monoclonal antibody specifically detects CD11b in FACS, IP and FM. It exhibits reactivity toward Human.

Quick Overview for CD11b antibody (PE) (ABIN6655311)

Target: CD11b (ITGAM) (Integrin alpha M (ITGAM))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD11b antibody is conjugated to PE

Application: Flow Cytometry (FACS), Immunoprecipitation (IP), Fluorescence Microscopy (FM)

Clone: ICRF44

Product Details anti-CD11b Antibody (PE)

Supplier Product No.: 200-308-n20

Supplier: Rockland

Purpose: CD11b Phycoerythrin Antibody

Cross-Reactivity (Details): Reactivity is observed against human CD11b, Baboon, Chimpanzee, Cynomolgus, Rhesus, and Swine.

Purification: Phycoerythrin conjugated CD11b Monoclonal Antibody was purified from tissue culture supernatant via affinity chromatography and is directed against human CD11b.

Sterility: Sterile filtered

Immunogen: Anti-CD11b Antibody (Monoclonal) was produced by repeated immunizations with CD11b antigen.

Isotype: IgG1 kappa

Labeling Ratio: 1-2

Application Details

Application Notes:

Immunoprecipitation_Dilution: User Optimized

Flow_Cytometry_Dilution: 5μL/test/1x10e5 to 1x10e8 cells

IF_Microscopy_Dilution: User Optimized

Comment:

Anti-CD11b is tested for Flow Cytometry and is useful in Immunofluorescence and Immunoprecipitation. Researchers should determine optimal titers for applications that are not stated.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer:

Buffer: 0.01 M Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 0.1 % Gelatin

Preservative: 0.09 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Store vial at 4° C prior to opening. Dilute only prior to immediate use. This product is stable at 4° C as an undiluted liquid. Use subdued lighting during handling and incubation of cells prior to analysis. Store reagent in the dark. DO NOT FREEZE.

Expiry Date: 6 months

Target Details for CD11b

Target: CD11b (ITGAM) (Integrin alpha M (ITGAM))

Alternative Name: CD11b

Background:

Synonyms: Integrin alpha-M, CD11 antigen-like family member B, CR-3 alpha chain, Cell surface glycoprotein MAC-1 subunit alpha, Leukocyte adhesion receptor MO1, Neutrophil adherence receptor, CD11b, CR3A

Background: The ICRF44 antibody reacts with human CD11b, also known as integrin alpha M. This 165-170 kDa cell surface glycoprotein is part of a family of integrin receptors that mediate adhesion between cells (cell-cell) and components of the extracellular matrix, e.g. fibrinogen (cell-matrix). In addition, integrins are active signaling receptors which recruit leukocytes to inflammatory sites and promote cell activation. Complete, functional integrin receptors consist of distinct combinations of integrin chains which are differentially expressed. Integrin alpha M (CD11b) assembles with Integrin beta-2 (CD18) into a receptor known as Macrophage Antigen-1 (Mac-1) or complement receptor type 3 (CR3). This receptor binds and induces intracellular signaling through ICAM-1, ICAM-2, ICAM-3 and ICAM-4 on endothelial cells and can also facilitate removal of iC3b bearing foreign cells.The ICRF44 antibody is widely used as a marker for CD11b expression on macrophages, granulocytes, and subsets of NK cells.

Gene Name: ITGAM

Gene ID: 3684

NCBI Accession: NP_000623

UniProt: P11215

Pathways: Apoptosis, Activation of Innate immune Response, Toll-Like Receptors Cascades, Activated T Cell Proliferation