TLR8 antibody

Catalog No. ABIN6655316

Supplier Product No.: 200-301-i30

Product Details anti-TLR8 Antibody

Target: TLR8 (Toll-Like Receptor 8 (TLR8))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This TLR8 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS), Dot Blot (DB)

Supplier Product No.: 200-301-i30

Supplier: Rockland

Purpose: TLR8 Antibody

Cross-Reactivity (Details): A BLAST analysis was used to suggest cross-reactivity with Anti-TLR8 from human and mouse based on 100 % homology with the immunizing sequence.

Purification: Anti-TLR8 Antibody was purified by Protein G chromatography.

Immunogen: TLR8 Antibody was produced in mice prepared by repeated immunizations with amino acids corresponding to an internal sequence of human TLR8 protein.

Clone: 44C143

Isotype: IgG1 kappa

Application Details

Application Notes:

Immunohistochemistry_Dilution: 5 μg/mL

Flow_Cytometry_Dilution: 0.5-1 μg/10^6 cells

Western_Blot_Dilution: 1-3 μg/mL

Comment:

Anti-TLR8 Antibody is tested for use in WB, DB, Flow, Flow-CS, Flow-IC, ICC/IF, IHC, and IHC-P. Expect a band approximately 121kDa on specific lysates. Specific conditions for reactivity should be optimized by the end user.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 0.05 % BSA

Preservative: 0.05 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Expiry Date: 12 months

Target Details for TLR8

Target: TLR8 (Toll-Like Receptor 8 (TLR8))

Alternative Name: TLR8 (TLR8 Products)

Background:

Synonyms: Toll-like receptor 8

Background: Anti-TLR8 CD288 Antibody detects human TLR8. The Toll-like receptor (TLR) family in mammal comprises a family of transmembrane proteins characterized by multiple copies of leucine rich repeats in the extracellular domain and IL-1 receptor motif in the cytoplasmic domain. Like its counterparts in Drosophila, TLRs signal through adaptor molecules. The TLR family is a phylogenetically conserved mediator of innate immunity that is essential for microbial recognition. Ten human homologs of TLRs (TLR1-10) have been described. TLR8 gene contains three exons, two of which have coding function. TLR8 cDNA codes for a protein of approximate molecular weight of 120 kDa. Anti-TLR8 CD288 Antibody is ideal for investigators involved in cytokines and growth factor research.

Gene Name: TLR8

Gene ID: 51311

NCBI Accession: NP_619542

UniProt: Q9NR97

Pathways: TLR Signaling, Activation of Innate immune Response, Toll-Like Receptors Cascades