TLR8 antibody

Catalog No. ABIN6655316

Product Details anti-TLR8 Antibody

Target: TLR8 (Toll-Like Receptor 8 (TLR8))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This TLR8 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Flow Cytometry (FACS)

Purification: Anti-TLR8 Antibody was purified by Protein G chromatography. A BLAST analysis was used to suggest cross-reactivity with Anti-TLR8 from human and mouse based on 100% homology with the immunizing sequence. Cross-reactivity with Anti-TLR8 from other sources has not been determined.

Immunogen:

Immunogen: TLR8 Antibody was produced in mice prepared by repeated immunizations with amino acids corresponding to an internal sequence of human TLR8 protein.

Immunogen Type: Peptide

Clone: 44C143

Isotype: IgG1

Application Details

Application Notes:

Immunohistochemistry Dilution: 5 μg/mL

Application Note: Anti-TLR8 Antibody is suitable for use in Flow (CS), Flow (I), IHC (p) and WB. Expect a band approximately 121 kDa on specific lysates. Specific conditions for reactivity should be optimized by the end user.

Flow Cytometry Dilution: 0.5-1 μg/10^6 cells

Western Blot Dilution: 1-3 μg/mL

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 0.05 % BSA

0.05 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: RT,4 °C,-20 °C

Storage Comment: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Target Details for TLR8

Target: TLR8 (Toll-Like Receptor 8 (TLR8))

Alternative Name: TLR8 (TLR8 Products)

Synonyms: CD288 antibody, toll like receptor 8 antibody, toll-like receptor 8 antibody, TLR8 antibody, Tlr8 antibody

Background:

Synonyms: Toll-like receptor 8

Background: Anti-TLR8 CD288 Antibody detects human TLR8. The Toll-like receptor (TLR) family in mammal comprises a family of transmembrane proteins characterized by multiple copies of leucine rich repeats in the extracellular domain and IL-1 receptor motif in the cytoplasmic domain. Like its counterparts in Drosophila, TLRs signal through adaptor molecules. The TLR family is a phylogenetically conserved mediator of innate immunity that is essential for microbial recognition. Ten human homologs of TLRs (TLR1-10) have been described. TLR8 gene contains three exons, two of which have coding function. TLR8 cDNA codes for a protein of approximate molecular weight of 120 kDa. Anti-TLR8 CD288 Antibody is ideal for investigators involved in cytokines and growth factor research.

Gene Name: TLR8

Gene ID: 51311

NCBI Accession: NP_619542

UniProt: Q9NR97

Pathways: TLR Signaling, Activation of Innate immune Response, Toll-Like Receptors Cascades