IKBKB antibody

Catalog No. ABIN6655866

Supplier Product No.: 200-301-h83

This Mouse Monoclonal antibody specifically detects IKBKB in WB, IHC, IF, IP and FACS. It exhibits reactivity toward Human.

Quick Overview for IKBKB antibody (ABIN6655866)

Target: IKBKB (Inhibitor of kappa Light Polypeptide Gene Enhancer in B-Cells, Kinase beta (IKBKB))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This IKBKB antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunoprecipitation (IP), Flow Cytometry (FACS)

Clone: 10AG2

Product Details anti-IKBKB Antibody

Supplier Product No.: 200-301-h83

Supplier: Rockland

Purpose: IKK beta Antibody

Cross-Reactivity (Details): A BLAST analysis was used to suggest cross-reactivity with IKKß from Human and Mouse based on 100 % homology with the immunizing sequence.

Purification: Anti-IKKß Antibody was purified by Protein G chromatography.

Immunogen: IKK beta Antibody was produced in mice prepared by repeated immunizations by a full-length human IKKbeta recombinant protein.

Isotype: IgG1

Application Details

Application Notes:

Immunoprecipitation_Dilution: 1:10 - 1:500

Immunohistochemistry_Dilution: 1:10 - 1:500

Flow_Cytometry_Dilution: 0.25-0.5 μg/10^6 cells

Western_Blot_Dilution: 2-4 μg/mL

Comment:

Anti-IKKb IKK2 Antibody is tested for use in WB, Flow, ICC/IF, IHC, IHC-P, and IP. Expect a band approximately 85kDa on specific lysates. Specific conditions for reactivity should be optimized by the end user.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Optional[Buffer]: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 ,0.05 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Expiry Date: 12 months

Target Details for IKBKB

Target: IKBKB (Inhibitor of kappa Light Polypeptide Gene Enhancer in B-Cells, Kinase beta (IKBKB))

Alternative Name: IKK beta

Background:

Synonyms: IKKB, Inhibitor of nuclear factor kappa-B kinase subunit beta, I-kappa-B-kinase beta, IKK-B, IKK-beta, IkBKB, 2.7.11.10, I-kappa-B kinase , IKK2, Nuclear factor NF-kappa-B inhibitor kinase beta, NFKBIKB

Background: Anti-IKKb IKK2 Antibody detects human IKKb IKK2. NF-kB (nuclear factor kB) is sequestered in the cytoplasm by IkB family of inhibitory proteins that mask the nuclear localization signal of NF-kB thereby preventing translocation of NF-kB to the nucleus. External stimuli such as tumor necrosis factor or other cytokines results in phosphorylation and degradation of IkB releasing NF-kB dimers. NF-kB dimer subsequently translocates to the nucleus and activates target genes. Synthesis of IkBa is autoregulated. IkB proteins are phosphorylated by IkB kinase complex consisting of at least three proteins, IKKa, IKKb (IKKbeta), and IKKg. IKKa and IKKbeta are phosphorylated by NF-kB-inducing kinase (NIK) and MAP kinase kinase kinase-1 (MEKK1), respectively. Recent studies have shown that IKKbeta, not IKKa, is the target proinflammatory stimuli. Targeted disruption of IKKbeta gene in mice also results in extensive liver damage from apoptosis and death as embryo. Anti-IKKb IKK2 Antibody is ideal for investigators involved in NFkaapB and cytokines and growth factors.

Gene Name: IKBKB

Gene ID: 3551

NCBI Accession: NP_001177649

UniProt: O14920

Pathways: NF-kappaB Signaling, RTK Signaling, TCR Signaling, TLR Signaling, Fc-epsilon Receptor Signaling Pathway, Neurotrophin Signaling Pathway, Activation of Innate immune Response, Production of Molecular Mediator of Immune Response, Hepatitis C, Toll-Like Receptors Cascades, BCR Signaling, Ubiquitin Proteasome Pathway, S100 Proteins