NOXA antibody

Catalog No. ABIN6655871

Supplier Product No.: 200-301-h98

This anti-NOXA antibody is a Mouse Monoclonal antibody detecting NOXA in WB, IF, IHC and FACS. Suitable for Human. This Primary Antibody has been cited in 2+ publications.

Quick Overview for NOXA antibody (ABIN6655871)

Target: NOXA (PMAIP1) (Phorbol-12-Myristate-13-Acetate-Induced Protein 1 (PMAIP1))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This NOXA antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (IHC), Flow Cytometry (FACS)

Clone: 114C307-1

Product Details anti-NOXA Antibody

Supplier Product No.: 200-301-h98

Supplier: Rockland

Purpose: NOXA Antibody

Cross-Reactivity (Details): A BLAST analysis was used to suggest cross-reactivity with Anti-Noxa from human and mouse based on 100 % homology with the immunizing sequence.

Purification: Anti-Noxa Antibody was purified by Protein G chromatography.

Immunogen: Noxa Antibody was produced in mice prepared by repeated immunizations with the human protein Noxa.

Isotype: IgG1 kappa

Application Details

Application Notes:

Immunohistochemistry_Dilution: 5 μg/mL

Western_Blot_Dilution: 1-2 μg/mL

Comment:

Anti-Noxa antibody is tested for use by WB, Flow, ICC/IF, IHC, and IHC-P. Expect a band approximately 6 kDa on specific lysates. Specific conditions for reactivity should be optimized by the end user.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Optional[Buffer]: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 ,0.05 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Expiry Date: 12 months

References for anti-NOXA antibody (ABIN6655871)

Haschka, Karbon, Soratroi, O'Neill, Luo, Villunger: "MARCH5-dependent degradation of MCL1/NOXA complexes defines susceptibility to antimitotic drug treatment." in: Cell death and differentiation, Vol. 27, Issue 8, pp. 2297-2312, (2021) (PubMed).

Ichikawa, Alves, Pfeiffer, Langa, Hernández-Santana, Suzuki, Prehn, Engel, Henshall: "Deletion of the BH3-only protein Noxa alters electrographic seizures but does not protect against hippocampal damage after status epilepticus in mice." in: Cell death & disease, Vol. 8, Issue 1, pp. e2556, (2017) (PubMed).

Target Details for NOXA

Target: NOXA (PMAIP1) (Phorbol-12-Myristate-13-Acetate-Induced Protein 1 (PMAIP1))

Alternative Name: Noxa

Background:

Synonyms: NOXA, Phorbol-12-myristate-13-acetate-induced protein 1, PMA-induced protein 1, Immediate-early-response protein APR, Protein Noxa

Background: Anti-Noxa antibody detects human Noxa. Recently, a BH-3 only member of the Bcl-2 family have been identified in human and mouse and designated as Noxa (for damage). The expression of the Noxa gene involves direct activation of its promoter by p53. Increased expression of Noxa protein occurs in normal thymocytes but not in p53-deficient thymocytes. Noxa cDNA codes for a 103-amino acid protein. The coimmunoprecipitation data suggest that Noxa protein may interact with proteins belonging to the Bcl-2 family, such as, Bcl-XL and Mcl-1. Oda et al. have also shown that blocking the endogenous Noxa induction results in the suppression of apoptosis. Treatment of cells with Noxa antisense oligonucleotides blocks radiation-induced apoptosis suggesting that Noxa may represent a mediator of p53-dependent apoptosis. Anti-Noxa antibody is ideal for investigators involved in apaoptosis research.

Gene Name: PMAIP1

Gene ID: 5366

NCBI Accession: NP_066950

UniProt: Q13794

Pathways: p53 Signaling, Positive Regulation of Endopeptidase Activity, Regulation of Carbohydrate Metabolic Process, Positive Regulation of Response to DNA Damage Stimulus