Vimentin antibody

Catalog No. ABIN6656056

Supplier Product No.: 200-301-e54

The Mouse Monoclonal anti-Vimentin antibody is suitable to detect Vimentin in samples from Human. It has been validated for WB, IHC, ELISA and FM.

Quick Overview for Vimentin antibody (ABIN6656056)

Target: Vimentin (VIM)

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This Vimentin antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Fluorescence Microscopy (FM)

Clone: 2D1

Product Details anti-Vimentin Antibody

Supplier Product No.: 200-301-e54

Supplier: Rockland

Purpose: Vimentin Antibody

Purification: Anti-Vimentin Antibody was purified from tissue culture supernatant by protein G chromatography.

Sterility: Sterile filtered

Immunogen: Anti-Vimentin Antibody was produced in mice by repeated immunizations with a recombinant human vimentin purified from E. coli.

Isotype: IgG2a

Application Details

Application Notes:

ELISA_Dilution: 1:10,000

Immunohistochemistry_Dilution: 1:500-1:1000

IF_Microscopy_Dilution: 1:500

Western_Blot_Dilution: 1:1000

Other: ICC: 1:500-1:2000

Comment:

Anti-Vimentin Antibody is tested for Western Blot, immunocytochemistry, and immunohistochemistry, and it is specific for the ~50 kDa vimentin protein. Specific conditions for reactivity should be optimized by the end user.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store vial at -20° C prior to opening in undiluted aliquots. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature.

Expiry Date: 12 months

Target Details for Vimentin

Target: Vimentin (VIM)

Alternative Name: Vimentin

Background:

Synonyms: Vim, Vimentin Antibody

Background: Vimentin is the major protein subunit of the 10nm or intermediate filaments (IFs) found in many kinds of mesenchymal and epithelial cells as well as developing neuronal and astrocytic precursor cells in the CNS. Vimentin is thought to be critically involved in lymphocyte adhesion and transmigration. Copolymers are frequently formed between vimentin and other IFs, such as GFAP (in many kinds of astrocytes), desmin (in muscle cells) and neurofilament proteins (in developing neurons). Antibodies to vimentin are useful in studies of stem cells and generally to reveal the filamentous cytoskeleton. Recent studies suggest that vimentin affects prostate cancer cells motility and invasiveness. Vimentin Antibody is ideal for researchers interested in neuroscience research.

Gene Name: VIM

Gene ID: 7431

NCBI Accession: NP_003371

UniProt: P08670

Pathways: Caspase Cascade in Apoptosis