Choline Acetyltransferase antibody

Catalog No. ABIN6656077

Product Details anti-Choline Acetyltransferase Antibody

Target: Choline Acetyltransferase (CHAT) (Choline O-Acetyltransferase (CHAT))

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This Choline Acetyltransferase antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Cross-Reactivity: Bird (Avian), Chicken, Guinea Pig, Human, Mouse (Murine), Non-Human Primate

Cross-Reactivity (Details): Cross reactivity with ChAT from other species has not been determined

Purification: Anti-ChAT antibody is directed against human ChAT protein. The antibody was affinity purified from monospecific antiserum by immunoaffinity purification. . However, reactivity is also expected from the following species based on 100% sequence homology: chicken, guinea pig, mouse, and non-human primates.

Immunogen:

Immunogen: Anti-ChAT Antibody was produced in rabbit by repeated immunizations with native choline acetyltransferase purified from human placenta.

Immunogen Type: Native Protein

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry Dilution: 1:100

Application Note: Anti-ChAT antibody is suitable for use in ELISA, Western Blotting and IHC. Specific conditions for reactivity should be optimized by the end user. Expect a ~ 68/70 kDa band corresponding to choline acetyltransferase protein by Western Blot in the appropriate lysate or tissue.

Western Blot Dilution: 1:100

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer:

Buffer: 0.01 M Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 5 mg BSA

0.02 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store vial at -20° C prior to opening.  This product is stable at 4° C as an undiluted liquid.  For extended storage, aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing. Dilute only prior to immediate use.

References for anti-Choline Acetyltransferase antibody (ABIN6656077)

Liu, Kim, Kim, Zhang, Bao, Denaxa, Lim, Kim, Liu, Wickersham, Pachnis, Hattar, Song, Brown, Blackshaw: "Lhx6-positive GABA-releasing neurons of the zona incerta promote sleep." in: Nature, Vol. 548, Issue 7669, pp. 582-587, (2017) (PubMed).

Target Details for Choline Acetyltransferase

Target: Choline Acetyltransferase (CHAT) (Choline O-Acetyltransferase (CHAT))

Alternative Name: ChAT (Choline Acetyltransferase) (CHAT Products)

Synonyms: CHOACTASE antibody, CMS1A antibody, CMS1A2 antibody, ChoAcTase antibody, B230380D24Rik antibody, choline O-acetyltransferase antibody, choline O-acetyltransferase a antibody, choline acetyltransferase antibody, carnitine O-acetyltransferase antibody, CHAT antibody, chata antibody, Chat antibody, PGTG_00551 antibody, LOC100533322 antibody

Background:

Synonyms: acetyl CoA:choline O-acetyltransferase, CHAT, CHOACTase, Choline acetylase, choline acetyltransferase, Choline O-acetyltransferase, CLAT, CMS1A, CMS1A2

Background: ChAT antibody recognizes choline acetyltransferase which is a neuronal enzyme which catalyzes the reaction between Acetyl CoA and choline resulting in the formation of acetylcholine. ChAT is found primarily in cholinergic neurons making it a valuable marker for diseases associated with decreased cholinergic function such as Schizophrenia, Alzheimer disease (AD) and Down syndrome. Decreased choline acetyltransferase activity in particular has been shown in Schizophrenic subjects. It has furthermore been demonstrated that in patients with AD, there are significantly lower levels of cortical ChAT that correlate with severity of the disease as measured by loss of neuropsychological function. Anti-ChAT antibody is ideal for investigators involved in Cell Signaling, Neuroscience, Signal Transduction research.

Gene Name: CHAT

Gene ID: 1103

UniProt: P28329

Pathways: Skeletal Muscle Fiber Development