Aquaporin 4 antibody (C-Term)

Catalog No. ABIN6656113

Product Details anti-Aquaporin 4 Antibody

Target: Aquaporin 4 (AQP4)

Binding Specificity: C-Term

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Aquaporin 4 antibody is un-conjugated

Application: Western Blotting (WB), Fluorescence Microscopy (FM)

Cross-Reactivity: Human, Mouse (Murine), Rat (Rattus)

Purification: Anti-Aquaporin 4 Antibody was purified by affinity chromatography. A BLAST analysis was used to suggest cross-reactivity with Aquaporin 4 from Human, Mouse, and Rat based on 100% homology with the immunizing sequence. Other species not yet tested. Cross-reactivity with Aquaporin 4 from other sources has not been determined. Channels and Transporters research.

Immunogen:

Immunogen: Aquaporin 4 Antibody was produced from whole rabbit serum prepared by repeated immunizations with a synthetic peptide corresponding to the C-terminal region.

Immunogen Type: Peptide

Isotype: IgG

Application Details

Application Notes:

Application Note: Anti-Aquaporin 4 Antibody is suitable for use in WB and IF microscopy. Expect a band approximately ~35 kDa on specific lysates. May detect larger glycosylated bands ~50 kDa. Specific conditions for reactivity should be optimized by the end user.

Western Blot Dilution: 1:1000-4000

IF Microscopy Dilution: 1:400

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 50 % (v/v) Glycerol

0.1 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: RT,4 °C,-20 °C

Storage Comment: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Target Details for Aquaporin 4

Target: Aquaporin 4 (AQP4)

Alternative Name: Aquaporin 4 (AQP4 Products)

Synonyms: zgc:100922 antibody, si:ch211-192k9.1 antibody, AQP4 antibody, LOC100232616 antibody, HMIWC2 antibody, MIWC antibody, WCH4 antibody, AQP-4 antibody, AQP4.M23 antibody, MIWC2 antibody, Miwc antibody, aquaporin-4 antibody, aquaporin 4 antibody, aquaporin 4 L homeolog antibody, aqp4 antibody, AQP4 antibody, aqp4.L antibody, Aqp4 antibody

Background:

Synonyms: AQP4, Aquaporin-4, Mercurial-insensitive water channel, MIWC, WCH4

Background: Aquaporins selectively conduct water molecules in and out of the cell, while preventing the passage of ions and other solutes. Known as water channels, they are integral membrane pore proteins. Aquaporin 4 is constitutively expressed in the basolateral cell membrane of princinpal collecting duct cells in the kidney and provide a pathway for water to exit these cells. It is also expressed in astrocytes and is upregulated by direct insult to the CNS. Aquaporin 4 has been propsed as the primary autoimmune target in neuromyelitis optica.

Gene Name: Aqp4

Gene ID: 25293

NCBI Accession: NP_036957

UniProt: P47863

Pathways: Sensory Perception of Sound