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GFAP R416WT antibody (AA 411-422) (HRP)

Reactivity: Human IHC, WB, ELISA Host: Mouse Monoclonal S206B-9 HRP
Catalog No. ABIN6656180
  • Target See all GFAP R416WT products
    GFAP R416WT
    Binding Specificity
    AA 411-422
    Reactivity
    • 7
    • 1
    • 1
    • 1
    Human
    Host
    • 8
    Mouse
    Clonality
    • 8
    Monoclonal
    Conjugate
    • 3
    • 1
    • 1
    • 1
    • 1
    • 1
    This GFAP R416WT antibody is conjugated to HRP
    Application
    • 8
    • 8
    • 6
    • 1
    • 1
    Immunohistochemistry (IHC), Western Blotting (WB), ELISA
    Cross-Reactivity
    Human, Mouse (Murine), Rat (Rattus)
    Purification
    Anti-GFAP R416WT Antibody was purified from concentrated tissue culture supernate by Protein G chromatography. A BLAST analysis was used to suggest cross-reactivity with rat and mouse based on 100% homology with the immunizing sequence. No cross-reactivity with other GFAP mutant proteins can be expected.
    Immunogen

    Immunogen: Anti-GFAP R416WT Antibody was produced in mice by repeated immunizations with a synthetic peptide corresponding to amino acids 411-422 (KTVEMRDGEVIK) of human GFAP.

    Immunogen Type: Peptide

    Clone
    S206B-9
    Isotype
    IgG1
  • Application Notes

    Immunohistochemistry Dilution: User Optimized

    Application Note: Anti-GFAP R416WT HRP Conjugated Antibody is suitable for use in Western blot, Immunohistochemistry, and Immunocytochemistry. Expect a band approximately ~50 kDa on specific lysates or tissues. Specific conditions for reactivity should be optimized by the end user.

    ELISA Dilution: 1:10,000

    Western Blot Dilution: 1:1000

    Restrictions
    For Research Use only
  • Format
    Liquid
    Buffer

    Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

    Stabilizer: 50 % (v/v) Glycerol

    0.1 % (w/v) Sodium Azide
    Preservative
    Sodium azide
    Precaution of Use
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Storage
    RT,4 °C,-20 °C
    Storage Comment
    Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
  • Target
    GFAP R416WT
    Abstract
    GFAP R416WT Products
    Background

    Synonyms: Glial fibrillary acidic protein, Intermediate filament protein, Astrocyte, gfapl, DKFZp459C0729, MGC139638, FLJ45472, AI836096, GFAP antibody

    Background: The 50 kDa type III intermediate filament protein glial fibrillary acidic protein (GFAP) is a major structural component of astrocytes. GFAP associates with the calcium binding protein annexin II-p2 and S-100. Association with these proteins together with phosphorylation regulates GFAP polymerization. Astroycytes respond to brain injury by proliferatin (astrogliosis), and one of the first events to occur during astrocyte proliferation is increased GFAP expression. Interestingly, antibodies to GFAP have been detected in individuals with dementia. Anti-GFAP is ideal for investigators involved in Neuroscience Research, including Alexander Disease, Oligodendroglioma, Cytoskleton Remolding Neurofilaments and PIP3/AKT Signaling.

    Gene Name: GFAP

    Gene ID
    2670
    UniProt
    P14136
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