ERK2 antibody (Internal Region) (DyLight 680)

Catalog No. ABIN6656700

Supplier Product No.: 200-344-gq1

This Mouse Monoclonal antibody specifically detects ERK2 in WB and ELISA. It exhibits reactivity toward Human.

Quick Overview for ERK2 antibody (Internal Region) (DyLight 680) (ABIN6656700)

Target: ERK2 (MAPK1) (Mitogen-Activated Protein Kinase 1 (MAPK1))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This ERK2 antibody is conjugated to DyLight 680

Application: Western Blotting (WB), ELISA

Clone: 3H9-A1-A8

Product Details anti-ERK2 Antibody (DyLight 680)

Binding Specificity: Internal Region

Supplier Product No.: 200-344-gq1

Supplier: Rockland

Purpose: ERK2 Internal Antibody DyLight™680

Cross-Reactivity (Details): This DyLight™680 conjugated protein A purified mouse monoclonal antibody reacts specifically with human ERK2.

Purification: Anti-ERK2 is purified from tissue culture supernatant by protein A purification.

Immunogen: Anti-ERK2 Monoclonal Antibody was produced in mice by repeated immunizations with synthetic peptide corresponding to amino acid residues near an internal region of ERK2 conjugated to KLH.

Isotype: IgG

Labeling Ratio: 3.0

Application Details

Application Notes:

ELISA_Dilution: 1:40,000

Western_Blot_Dilution: 1 μg/mL

Comment:

Anti-ERK 2 (MOUSE) DyLight™680 conjugated antibody is suitable for use in ELISA and Western Blotting. Specific conditions of reactivity should be optimized by the end user. Expect a band of approximately 41 kDa.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution:

Reconstitution_Buffer: Restore with deionized water (or equivalent)

Reconstitution_Volume: 100 μL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Preservative: 0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Expiry Date: 12 months

Target Details for ERK2

Target: ERK2 (MAPK1) (Mitogen-Activated Protein Kinase 1 (MAPK1))

Alternative Name: ERK2

Background:

Synonyms: mouse anti-ERK2 antibody DyLight™680 conjugation, DyLight™680 conjugated mouse anti-ERK2 antibody, MAPK1, ERK, ERK-2, ERK2, MAPK2, P42MAPK, PRKM1, PRKM2, DyLight™680, ERK-2 Antibody

Background: ERK2 antibodies detect the ERK2 isoform. Mitogen activated protein kinase 1, also known as MAPK1, ERK, or ERK2, is an integral component of the MAP kinase cascade that regulates cell growth and differentiation. ERK1 and ERK2 are activated by MEK1 and MEK2 in the B-raf signaling pathway resulting in its translocation to the nucleus where it phosphorylates nuclear targets. Human ERK1 and ERK2 are 84 % identical in sequence and share common functionality in cells. Anti-ERK2 antibody is ideal for investigators involved in Neuroscience, Cell Signaling and Cancer Research.

Gene Name: MAPK1

UniProt: P28482

Pathways: MAPK Signaling, RTK Signaling, Apoptosis, Interferon-gamma Pathway, Fc-epsilon Receptor Signaling Pathway, Response to Growth Hormone Stimulus, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Hepatitis C, Protein targeting to Nucleus, Toll-Like Receptors Cascades, Monocarboxylic Acid Catabolic Process, Autophagy, G-protein mediated Events, Signaling Events mediated by VEGFR1 and VEGFR2, Signaling of Hepatocyte Growth Factor Receptor, VEGFR1 Specific Signals, BCR Signaling, S100 Proteins