GPBAR1 antibody (C-Term)

Catalog No. ABIN6657009

Product Details anti-GPBAR1 Antibody

Target: GPBAR1 (G Protein-Coupled Bile Acid Receptor 1 (GPBAR1))

Binding Specificity: C-Term

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This GPBAR1 antibody is un-conjugated

Application: Western Blotting (WB)

Cross-Reactivity: Human, Mouse (Murine), Rat (Rattus)

Purification: Anti-BG37 Antibody is sera. A BLAST analysis was used to suggest cross-reactivity with BG37 from human and mouse based on 100% homology with the immunizing sequence. Predicted reactivity with cow. Cross-reactivity with BG37 from other sources has not been determined.

Immunogen:

Immunogen: BG37 Antibody was produced from whole rabbit serum which was prepared by repeated immunizations with the amino acid portion of human BG37 near the C-terminus of the chain .

Immunogen Type: Peptide

Application Details

Application Notes:

Application Note: Anti-BG37 Antibody is suitable for use in WB. Expect a band approximately 37 kDa on specific lysates. Specific conditions for reactivity should be optimized by the end user.

Western Blot Dilution: 1:1000-1:5000

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: 50 % (v/v) Glycerol0.05 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: RT,4 °C,-20 °C

Storage Comment: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Target Details for GPBAR1

Target: GPBAR1 (G Protein-Coupled Bile Acid Receptor 1 (GPBAR1))

Alternative Name: BG37 (GPBAR1 Products)

Synonyms: BG37 antibody, GPCR19 antibody, GPR131 antibody, M-BAR antibody, TGR5 antibody, Tgr5 antibody, MGC84403 antibody, GPBAR1 antibody, Gpbar1 antibody, GPCR antibody, G protein-coupled bile acid receptor 1 antibody, G protein-coupled bile acid receptor 1 S homeolog antibody, GPBAR1 antibody, Gpbar1 antibody, gpbar1.S antibody

Background:

Synonyms: TGR5, G-protein coupled bile acid receptor 1, G-protein coupled receptor GPCR19, hGPCR19, Membrane-type receptor for bile acids, M-BAR, hBG37, BG37

Background: Anti-BG37 TGR5 Antibody detects human BG37tgr5. BG37 is a 330 amino acid novel enzyme with seven transmembrane domains. It belongs to the G protein-coupled receptor (GPCR) superfamily and functions as a cell surface receptor for bile acids. Cells expressing BG37 when treated with bile acids induce the production of intracellular cAMP, activate the MAP kinase signaling pathway, leads to the internalization of the receptor and regulates proglucagon gene expression in enteroendocrine cells. The receptor is implicated in the suppression of macrophage functions and regulation of energy homeostasis by bile acids that can be targeted to improve metabolic control. It is known to be ubiquitously expressed in most of the tissues. Anti-BG37 TGR5 Antibody is ideal for investigators involved in kinase, phosphatase, and NFkappaB research.

Gene Name: GPBAR1

Gene ID: 151306

NCBI Accession: NP_001070659

UniProt: Q8TDU6

Pathways: WNT Signaling, Hormone Transport, Sensory Perception of Sound