Choline Acetyltransferase antibody

Catalog No. ABIN6657460

Supplier Product No.: 100-401-d16

The Rabbit Polyclonal anti-Choline Acetyltransferase antibody has been validated for WB and IHC. It is suitable to detect Choline Acetyltransferase in samples from Human.

Quick Overview for Choline Acetyltransferase antibody (ABIN6657460)

Target: Choline Acetyltransferase (CHAT) (Choline O-Acetyltransferase (CHAT))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Choline Acetyltransferase antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-Choline Acetyltransferase Antibody

Supplier Product No.: 100-401-d16

Supplier: Rockland

Purpose: ChAT (Choline Acetyltransferase) Antibody

Cross-Reactivity (Details): Reactivity against ChAT occurs from human sources.

Purification: Anti-ChAT antibody is neat antiserum prepared from monospecific antiserum by delipidation and defibrination.

Immunogen: Anti-ChAT Antibody was produced in rabbit by repeated immunizations with native choline acetyltransferase purified from human placenta

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry_Dilution: 1:100

Western_Blot_Dilution: 1:1000

Comment:

Anti-ChAT antibody has been tested in western blot and IHC and is suitable for use in ELISA. Specific conditions for reactivity should be optimized by the end user. Expect a ~ 68/70 kDa band corresponding to choline acetyltransferase protein by Western Blot in the appropriate lysate or tissue.

Restrictions: For Research Use only

Handling

Format: Liquid

Storage: 4 °C,-20 °C

Storage Comment: Store vial at -20° C prior to opening.  This product is stable at 4° C as an undiluted liquid.  For extended storage, aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing. Dilute only prior to immediate use.

Expiry Date: 12 months

Target Details for Choline Acetyltransferase

Target: Choline Acetyltransferase (CHAT) (Choline O-Acetyltransferase (CHAT))

Alternative Name: ChAT (Choline Acetyltransferase)

Background:

Synonyms: acetyl CoA:choline O-acetyltransferase, CHAT, CHOACTase, Choline acetylase, choline acetyltransferase, Choline O-acetyltransferase, CLAT, CMS1A, CMS1A2

Background: ChAT antibody recognizes choline acetyltransferase which is a neuronal enzyme which catalyzes the reaction between Acetyl CoA and choline resulting in the formation of acetylcholine. ChAT is found primarily in cholinergic neurons making it a valuable marker for diseases associated with decreased cholinergic function such as Schizophrenia, Alzheimer disease (AD) and Down syndrome. Decreased choline acetyltransferase activity in particular has been shown in Schizophrenic subjects. It has furthermore been demonstrated that in patients with AD, there are significantly lower levels of cortical ChAT that correlate with severity of the disease as measured by loss of neuropsychological function. Anti-ChAT antibody is ideal for investigators involved in Cell Signaling, Neuroscience, Signal Transduction research.

Gene Name: CHAT

Gene ID: 1103

UniProt: P28329

Pathways: Skeletal Muscle Fiber Development