p53 antibody (pSer392)

Catalog No. ABIN6657462

Supplier Product No.: 600-401-e04

This Rabbit Polyclonal antibody specifically detects p53 in WB. It exhibits reactivity toward Human.

Quick Overview for p53 antibody (pSer392) (ABIN6657462)

Target: p53 (TP53) (Tumor Protein P53 (TP53))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This p53 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-p53 Antibody

Binding Specificity: pSer392

Supplier Product No.: 600-401-e04

Supplier: Rockland

Purpose: p53 phospho S392 Antibody

Cross-Reactivity (Details): Anti-p53 pS392 antibody is directed against p53 phosphorylated at S392.

Purification: The antibody was prepared from monospecific antiserum by immunoaffinity chromatography using phospho peptide coupled to agarose beads followed by solid phase adsorption(s) against non-phospho peptide and non-specific peptide to remove any unwanted reactivities.

Immunogen: Anti-p53 pS392 Antibody was produced by repeated immunizations with a synthetic phospho-peptide corresponding to amino acid residues surrounding Ser392.

Isotype: IgG

Application Details

Application Notes:

Western_Blot_Dilution: 1:1000

Other: User Optimized

Comment:

Anti-p53 pS392 Antibody is tested for use in Western Blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band of approximately 53 kDa in size corresponding to p53 protein phosphorylated at Ser392 in the appropriate cell lysate or extract.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer:

Buffer: 0.01 M HEPES, 0.15 M Sodium Chloride, pH 7.5

Stabilizer: 0.1 mg/mL Bovine Serum Albumin (BSA) - IgG and Protease free, 50 % (v/v) Glycerol

Storage: 4 °C,-20 °C

Storage Comment: Store vial at -20° C prior to opening.  This product is stable at 4° C as an undiluted liquid.  For extended storage, aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing. Dilute only prior to immediate use.

Expiry Date: 12 months

Target Details for p53

Target: p53 (TP53) (Tumor Protein P53 (TP53))

Alternative Name: p53

Background:

Synonyms: Cellular tumor antigen p53, Antigen NY-CO-13, Phosphoprotein p53, Tumor suppressor p53

Background: p53 pS392 Antibody detects phophorylated p53. p53 has a well established role in blocking the proliferative action of damaged cells and acting in essence as an anticancer agent. It has been called the guardian of the genome. Phosphorylation of Ser392 in p53 is associated with formation of human tumors. In addition, p53 has been linked to effects of aging and oxidative stress. An increase in p53 has also been linked to deficits in LTP and learning and memory. Anti-p53 pS392 Antibody is ideal for investigators involved in Transcription Factor, Cell Cycle, Apoptosis, and Cancer research.

Gene Name: TP53

Gene ID: 7157

UniProt: P04637

Pathways: p53 Signaling, MAPK Signaling, PI3K-Akt Signaling, Apoptosis, AMPK Signaling, Chromatin Binding, ER-Nucleus Signaling, Positive Regulation of Endopeptidase Activity, Hepatitis C, Protein targeting to Nucleus, Autophagy, Warburg Effect