Talin antibody

Catalog No. ABIN6657478

Product Details anti-Talin Antibody

Target: Talin (TLN)

Reactivity: Human, Cow

Host: Mouse

Clonality: Monoclonal

Conjugate: This Talin antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Cross-Reactivity: Cow (Bovine), Human

Cross-Reactivity (Details): Cross reactivity with Talin from other sources has not been determined.

Purification: Anti-Talin antibody was purified from mouse ascites by protein A chromatography.  This product reacts with human and bovine.

Immunogen:

Immunogen: Talin antibody was produced in mice by repeated immunizations with purified chicken gizzard talin.

Immunogen Type: Other

Clone: Tal-33

Isotype: IgG1

Application Details

Application Notes:

Application Note: Anti-Talin is useful for Western Blot. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately ~270-272 kDa corresponding to the appropriate cell lysate or extract.

ELISA Dilution: 1:40,000

Western Blot Dilution: 2-4 μg/mL

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution:

Reconstitution Volume: 1.0 mL

Reconstitution Buffer: Neutral PBS

Buffer:

Buffer: 1.2 % sodium acetate

Stabilizer: 2 mg BSA

0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: RT,4 °C,-20 °C

Storage Comment: Store vial at 4° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Target Details for Talin

Target: Talin (TLN)

Alternative Name: Talin (TLN Products)

Background:

Synonyms: TLN, KIAA1027, ILWEQ, TALIN

Background: Talin is a high-molecular-weight cytoskeletal protein concentrated at regions of cell-substratum contact and, in lymphocytes, at cell-cell contacts. Talin serves a function of linking vinculin to the integrins, and, thus, the cytoskeleton to extracellular matrix (ECM) receptors. It has a mass of 270 kD and shares 23 % N-terminal identity with ezrin, which has similar functions. TLN gene is mapped to 9p. This antibody is suitable for researchers interested in cell signaling, membrane biology, and growth factor research.

Gene Name: TLN1, TLN2

Gene ID: 7094

NCBI Accession: NP_006280

UniProt: Q9Y490