RPS6 antibody (pSer244)

Catalog No. ABIN6657597

Supplier Product No.: 600-401-x26

This Rabbit Polyclonal antibody specifically detects RPS6 in WB and ELISA. It exhibits reactivity toward Mouse.

Quick Overview for RPS6 antibody (pSer244) (ABIN6657597)

Target: RPS6 (Ribosomal Protein S6 (RPS6))

Reactivity: Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This RPS6 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-RPS6 Antibody

Binding Specificity: pSer244

Supplier Product No.: 600-401-x26

Supplier: Rockland

Purpose: Ribosomal protein S6 Phospho S244 Antibody

Cross-Reactivity (Details): Anti-Phospho-Ser244 Ribosomal Protein S6 Antibody is directed against Ribosomal Protein S6 phosphorylated at S244.

Purification: The antibody was prepared from monospecific antiserum by immunoaffinity chromatography using phospho peptide coupled to agarose beads followed by solid phase adsorption(s) against non-phospho peptide and non-specific peptide to remove any unwanted reactivities.

Sterility: Sterile filtered

Immunogen: Anti-Phospho-Ser244 Ribosomal Protein S6 Antibody was produced in rabbits by repeated immunizations with a synthetic phospho-peptide corresponding to amino acid residues surrounding the phospho-Ser244 of mouse rpS6 conjugated to KLH.

Isotype: IgG

Application Details

Application Notes:

ELISA_Dilution: 1:10,000

Western_Blot_Dilution: 1:1000

Comment:

Anti-Ribosomal Protein S6 pS244 Antibody is tested for Western Blots and is specific for the ~28 kDa rpS6 protein phosphorylated at Ser244. Specific conditions for reactivity should be optimized by the end user.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer:

Buffer: 0.01 M HEPES, 0.15 M Sodium Chloride, pH 7.5

Stabilizer: 0.1 mg/mL Bovine Serum Albumin (BSA) - IgG and Protease free, 50 % (v/v) Glycerol

Preservative: None

Preservative: Without preservative

Storage: -20 °C

Storage Comment: Store vial at -20° C prior to opening in undiluted aliquots. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature.

Expiry Date: 12 months

Target Details for RPS6

Target: RPS6 (Ribosomal Protein S6 (RPS6))

Alternative Name: Ribosomal protein S6

Background:

Synonyms: 40S ribosomal protein S6, Phosphoprotein NP33, rpS6

Background: Ribosomal protein S6 (rpS6) is a critical component of the 40 S ribosomal subunit that mediates translation initiation at the 5'-m7GpppG cap of mRNA. The rpS6 protein is both cytoplasmic and nuclear localized . In response to mitogenic stimuli, rpS6 undergoes ordered C-terminal phosphorylation by p70 S6 kinases and p90 ribosomal S6 kinases on four Ser residues (Ser-235, Ser-236, Ser-240, and Ser-244) whose modification potentiates rpS6 cap binding activity. Additionally, rpS6 phosphorylation and function are highly regulated and have been implicated in the regulation of translational initiation and protein synthesis in response to extracellular stimuli such as TRAIL and gamma interferon (IFN-γ), as well as upon activation of the phosphatidylinositol 3-kinase (PI3K)-Akt-mTOR pathway. Ribosomal Protein S6 pS244 Antibody is ideal for researchers interested in neuroscience research.

Gene Name: RPS6

Gene ID: 20104

NCBI Accession: NP_033122

UniProt: P62754

Pathways: Carbohydrate Homeostasis, Ribonucleoprotein Complex Subunit Organization, Ribosome Assembly