KCNMB4 antibody (Extracellular Domain)

Catalog No. ABIN6657696

Product Details anti-KCNMB4 Antibody

Target: KCNMB4 (Potassium Large Conductance Calcium-Activated Channel, Subfamily M, beta Member 4 (KCNMB4))

Binding Specificity: Extracellular Domain

Reactivity: Mouse

Host: Mouse

Clonality: Monoclonal

Conjugate: This KCNMB4 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Fluorescence Microscopy (FM), Immunoprecipitation (IP)

Cross-Reactivity: Human, Mouse (Murine), Rat (Rattus)

Purification: Anti-BK Beta4 Antibody was purified by Protein G chromatography. A BLAST analysis was used to suggest cross-reactivity with BK Beta4 from Human, Mouse, and Rat based on 100% homology with the immunizing sequence. No cross-reactivity against BKBeta1, BKBeta2 or BKBeta3. Cross-reactivity with BK Beta4 from other sources has not been determined. Ion Channels research.

Immunogen:

Immunogen: BK Beta 4 Antibody was produced in mice by repeated immunizations with a fusion protein (entire extracellular domain) of mouse BKBeta4.

Immunogen Type: Recombinant Protein

Clone: S18A-3

Isotype: IgG2a

Application Details

Application Notes:

Immunohistochemistry Dilution: 0.1-1.0 μg/mL

Application Note: Anti-BK Beta4 Antibody is suitable for use in WB, IP, IF, and IHC. Antibody is provided in PBS pH 7.4. Expect a band approximately ~24 kDa on specific lysates. Specific conditions for reactivity should be optimized by the end user.

Immunoprecipitation Dilution: User Optimized

Western Blot Dilution: 1 μg/mL

IF Microscopy Dilution: 1.0-10 μg/mL

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer:

Buffer:

Stabilizer: 50 % (v/v) Glycerol

0.09 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: RT,4 °C,-20 °C

Storage Comment: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Target Details for KCNMB4

Target: KCNMB4 (Potassium Large Conductance Calcium-Activated Channel, Subfamily M, beta Member 4 (KCNMB4))

Alternative Name: BK Beta 4 (KCNMB4 Products)

Synonyms: 1700058G18Rik antibody, 2900045G12Rik antibody, kcnmb4 antibody, potassium calcium-activated channel subfamily M regulatory beta subunit 4 antibody, potassium large conductance calcium-activated channel, subfamily M, beta member 4 antibody, potassium channel subfamily M regulatory beta subunit 4 antibody, Kcnmb4 antibody, KCNMB4 antibody, kcnmb4 antibody

Background:

Synonyms: calcium-activated potassium channel subunit beta-4, BK channel subunit beta-4, BKbeta4, Calcium-activated potassium channel, subfamily M subunit beta-4, Charybdotoxin receptor subunit beta-4, K(VCA)beta-4, Maxi K channel subunit beta-4, Slo-beta-4

Background: BK channels contribute to electrical impulses, proper signal transmission of information and regulation of neurotransmitter release. A gain of function mutation in the pore-forming alpha subunit of the BK channel was linked to human neurological diseases. Findings suggest that the distribution of the beta subunits in the brain can modulate the BK channels to contribute to the pathophysiology of epilepsy and dyskinesia. This has major implications on other physiological processes in tissues other than the brain.

Gene Name: Kcnmb4

NCBI Accession: NP_067427