CD45.1 antibody (FITC)

Catalog No. ABIN6657896

Product Details anti-CD45.1 Antibody (FITC)

Target: CD45.1 (CD45 (CD45.1 Allogen))

Reactivity: Mouse

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD45.1 antibody is conjugated to FITC

Application: Flow Cytometry (FACS), Immunohistochemistry (IHC), Immunoprecipitation (IP)

Cross-Reactivity (Details): Cross reactivity with CD45.1 from other sources has not been tested

Purification: Fluorescein conjugated CD45.1 Monoclonal Antibody was purified from tissue culture supernatant via affinity chromatography and is directed against mouse CD45.1. . Anti-CD45.1 is conjugated with FITC under optimal conditions and the solution is free of unconjugated FITC.

Immunogen:

Immunogen: Anti-CD45.1 Antibody (Monoclonal) was produced by repeated immunizations with CD45.1 antigen.

Clone: A20

Isotype: IgG2a

Labeling Ratio: 2-8

Application Details

Application Notes:

Immunohistochemistry Dilution: User Optimized

Application Note: Anti-CD45.1 is useful for Flow Cytometry, Immunoprecipitation, and Immunohistochemistry using mouse spleen cells, or an appropriate cell type (where indicated). Researchers should determine optimal titers for applications that are not stated.

Immunoprecipitation Dilution: User Optimized

Flow Cytometry Dilution: 10 μL/10^6 cells (0.1 μg)

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer:

Buffer: 0.01 M Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 0.1 % Gelatin

0.09 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Store vial at 4° C prior to opening.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use.  DO NOT FREEZE. This product is light sensitive.

Target Details for CD45.1

Target: CD45.1 (CD45 (CD45.1 Allogen))

Alternative Name: CD45.1 (CD45.1 Products)

Background:

Synonyms: Receptor-type tyrosine-protein phosphatase C, , ukocyte common antigen, L-CA, Lymphocyte antigen 5, Ly-5, T200, CD45, Ptprc, Ly-5

Background: The A20 antibody reacts with mouse CD45.1, also known as Ly5.1, which is a strain-specific allelic form of the CD45 Leukocyte Common Antigen (LCA). Functionally, CD45 is a protein tyrosine phosphatase whose broad cell distribution supports a critical role in many leukocyte functions, including regulation of signal transduction and cell activation associated with the T cell and B cell receptors. The A20 antibody is typically used as a leukocyte marker in Ly5.1 mouse strains: SJL/J, DA, STS/A and RIII. The antibody has been demonstrated to specific for CD45.1 and is not cross-reactive with CD45.2 bearing cells.

Gene Name: Ptprc

UniProt: P06800