NEFM antibody (C-Term)
-
- Target See all NEFM Antibodies
- NEFM (Neurofilament Triplet M Protein (NEFM))
-
Binding Specificity
- C-Term
-
Reactivity
- Rat
-
Host
-
Chicken
-
Clonality
- Polyclonal
-
Conjugate
- This NEFM antibody is un-conjugated
-
Application
- Western Blotting (WB), Fluorescence Microscopy (FM)
- Cross-Reactivity
- Chicken, Cow (Bovine), Human, Mouse (Murine), Rat (Rattus)
- Cross-Reactivity (Details)
- Cross reactivity with NF-M from other species has not been determined.
- Purification
- Anti-NF-M antibody is directed against NF-M protein. The antibody is a total IgY fraction. Expect reactivity with the following species based on sequence homology: chicken, human, mouse.
- Immunogen
-
Immunogen: Anti-NF-M Monoclonal Antibody was produced by repeated immunizations with recombinant C-terminus part of NF-M expressed in E. coli.
Immunogen Type: Recombinant Protein
- Isotype
- IgG
- Top Product
- Discover our top product NEFM Primary Antibody
-
-
- Application Notes
-
Application Note: Anti- Neurofilament M antibody is suitable for use in Western Blotting and IF. Specific . Expect a band of approximately 145 kDa in size corresponding to NF-M proteins in the appropriate cell lysate or extract. Researchers should determine optimal titers for applications that are not stated below.
Western Blot Dilution: 1:5000
IF Microscopy Dilution: 1:1000
- Restrictions
- For Research Use only
-
- Format
- Liquid
- Buffer
- 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.20.01 % (w/v) Sodium Azide
- Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Storage
- 4 °C,-20 °C
- Storage Comment
- Store vial at -20° C prior to opening. This product is stable at 4° C as an undiluted liquid. For extended storage, aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Dilute only prior to immediate use.
-
-
Dynamic neuromuscular remodeling precedes motor-unit loss in a mouse model of ALS." in: eLife, Vol. 7, (2019) (PubMed).
: "Glial cells decipher synaptic competition at the mammalian neuromuscular junction." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 33, Issue 4, pp. 1297-313, (2013) (PubMed).
: "
-
Dynamic neuromuscular remodeling precedes motor-unit loss in a mouse model of ALS." in: eLife, Vol. 7, (2019) (PubMed).
-
- Target
- NEFM (Neurofilament Triplet M Protein (NEFM))
- Alternative Name
- Neurofilament M (NEFM Products)
- Synonyms
- NEF3 antibody, NF-M antibody, NFM antibody, Nef3 antibody, Nfm antibody, NF160 antibody, NF165 antibody, nef-m antibody, nef3 antibody, nefm antibody, nf-m antibody, zgc:112359 antibody, nefm-a antibody, nefm-b antibody, neurofilament medium antibody, neurofilament triplet M protein antibody, neurofilament, medium polypeptide antibody, neurofilament medium polypeptide antibody, neurofilament medium L homeolog antibody, neurofilament, medium polypeptide a antibody, neurofilament medium S homeolog antibody, NEFM antibody, Nefm antibody, TVAG_582010 antibody, TVAG_379630 antibody, nefm.L antibody, nefma antibody, nefm.S antibody
- Background
-
Synonyms: chicken anti-Neurofilament M antibody, Neurofilament medium polypeptide, NF-M, 160 kDa neurofilament protein, Neurofilament 3, Neurofilament triplet M protein
Background: NF-M antibody detects Neurofilament M protein. Neurofilaments are the 10nm or intermediate filament proteins found specifically in neurons, and are composed predominantly of three major proteins called NF-L, NF-M and NF-H. NF-M is the neurofilament middle or medium molecular weight polypeptide and runs on SDS-PAGE gels at 145-160 kDa, with some variability across species boundaries. Antibodies to NF-M are useful for identifying neuronal cells and their processes in tissue sections and in tissue culture. NF-M antibodies can also be useful to visualize neurofilament accumulations seen in many neurological diseases, such as Amyotrophic Lateral Sclerosis (Lou Gehrig's disease) and Alzheimer's disease. Neurofilament M Antibody is ideal for investigators involved in Cell Signaling and Neuroscience research.
Gene Name: NEFM
- Gene ID
- 24588
- UniProt
- P12839
- Pathways
- Brown Fat Cell Differentiation
-