GRIN2B antibody (C-Term)

Catalog No. ABIN6658060

Supplier Product No.: 600-401-c87

The Rabbit Polyclonal anti-GRIN2B antibody has been validated for WB, IHC and IP. It is suitable to detect GRIN2B in samples from Rat.

Quick Overview for GRIN2B antibody (C-Term) (ABIN6658060)

Target: GRIN2B (Glutamate Receptor, Ionotropic, N-Methyl D-Aspartate 2B (GRIN2B))

Reactivity: Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This GRIN2B antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP)

Product Details anti-GRIN2B Antibody

Binding Specificity: C-Term

Supplier Product No.: 600-401-c87

Supplier: Rockland

Purpose: NMDA NR2B Subunit Antibody

Cross-Reactivity (Details): Anti-NMDA 2B antibody is directed against NMDA receptor protein.

Purification: The antibody was affinity purified from monospecific antiserum by immunoaffinity purification.

Immunogen: Anti-NMDA NR2B Antibody was produced by repeated immunizations with a fusion protein from the C-terminal region NR2B subunit.

Isotype: IgG

Application Details

Application Notes:

Immunoprecipitation_Dilution: 3 μL per 200 μg lysate

Immunohistochemistry_Dilution: 1:1000-1:2000

Western_Blot_Dilution: 1:1000

Other: User Optimized

Comment:

Anti-NMDA NR2B subunit (Rabbit) antibody is tested for use in Western Blotting, Immunoprecipitation and IHC. Specific conditions for reactivity should be optimized by the end user. Expect a band or approximately 180 kDa in size corresponding to the NR2B subunit of the NMDA receptor in the appropriate cell lysate or extract.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution:

Reconstitution_Buffer: Neutral PBS

Reconstitution_Volume: 50μL

Storage: 4 °C,-20 °C

Storage Comment: Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. Dilute only prior to immediate use.

Expiry Date: 12 months

Target Details for GRIN2B

Target: GRIN2B (Glutamate Receptor, Ionotropic, N-Methyl D-Aspartate 2B (GRIN2B))

Alternative Name: NMDA NR2B Subunit

Background:

Synonyms: Glutamate [NMDA] receptor subunit epsilon-2, N-methyl D-aspartate receptor subtype 2B, NMDAR2B, NR2B, Grin2b

Background: NMDA 2B antibody detects NMDA 2B receptor protein. The ion channels activated by glutamate that are sensitive to N-methyl-D-aspartate (NMDA) are designated NMDA receptors (NMDAR). The NMDAR plays an essential role in memory, neuronal development and it has also been implicated in several disorders of the central nervous system including Alzheimer's, epilepsy and ischemic neuronal cell death. The NMDA receptor is also one of the principal molecular targets for alcohol in the CNS. The rat NMDAR1 (NR1) was the first subunit of the NMDAR to be cloned and it can form NMDA activated channels when expressed in Xenopus oocytes but the currents in such channels are much smaller than those seen in situ. Channels with more physiological characteristics are produced when the NR1 subunit is combined with one or more of the NMDAR2 (NR2 A-D) subunits. Overexpression of the NR2B-subunit of the NMDA receptor has been associated with increases in learning and memory while aged, memory impaired animals have deficiencies in NR2B expression. The NMDAR is also potentiated by protein phosphorylation. Anti-NMDA 2B antibody is ideal for investigators involved in Cell Signaling, Neuroscience, and Signal Transduction research

Gene Name: GRIN2B

Gene ID: 24410

NCBI Accession: NP_036706

UniProt: Q00960

Pathways: Response to Growth Hormone Stimulus, Synaptic Membrane, Feeding Behaviour, Regulation of long-term Neuronal Synaptic Plasticity