CDK5 antibody

Catalog No. ABIN6658103

Supplier Product No.: 200-301-163

This anti-CDK5 antibody is a Mouse Monoclonal antibody detecting CDK5 in WB and IHC. Suitable for Rat. This Primary Antibody has been cited in 4+ publications.

Quick Overview for CDK5 antibody (ABIN6658103)

Target: CDK5 (Cyclin-Dependent Kinase 5 (CDK5))

Reactivity: Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: This CDK5 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Clone: 1H3

Product Details anti-CDK5 Antibody

Supplier Product No.: 200-301-163

Supplier: Rockland

Purpose: Cdk5 Antibody

Cross-Reactivity (Details): Anti-CDK5 Antibody is directed against rat CDK5 protein.

Purification: The antibody was Protein G purified from cell culture supernatant by chromatography.

Immunogen: Anti-CDK5 Antibody was produced by repeated immunizations with purified rat Cdk5

Isotype: IgG1

Application Details

Application Notes:

Immunohistochemistry_Dilution: 1:500

Western_Blot_Dilution: 1:1000

Comment:

Suggested Applications: IF
Anti-CDK5 (Mouse) has been tested for use in Western Blotting and is excellent for use in IHC. Specific conditions for reactivity should be optimized by the end user. Expect a band of ~28kDa in size in rat striatal lysates by Western Blotting. Anti-CDK5 antibodies also work well for immunocytochemistry using primary cultured rat neurons and for immunohistochemistry on mouse brain tissue.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer:

Buffer: 0.01 M HEPES, 0.15 M Sodium Chloride, pH 7.5

Stabilizer: 0.1 mg/mL Bovine Serum Albumin (BSA) - IgG and Protease free, 50 % (v/v) Glycerol

Storage: 4 °C,-20 °C

Storage Comment: Store vial at -20° C prior to opening.  This product is stable at 4° C as an undiluted liquid.  For extended storage, aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing. Dilute only prior to immediate use.

Expiry Date: 12 months

References for anti-CDK5 antibody (ABIN6658103)

Gupta, Herring, Kumar, Telange, Garcia-Buntley, Caceres, Colantonio, Williams, Kurup, Carter, Lin, Chen, Rose, Jaskula-Sztul, Mukhtar, Reddy, Bibb: "Faulty Metabolism: A Potential Instigator of an Aggressive Phenotype in Cdk5-dependent Medullary Thyroid Carcinoma." in: bioRxiv : the preprint server for biology, (2023) (PubMed).

Thomas, Hernandez, Benavides, Li, Tan, Umfress, Plattner, Chakraborti, Pozzo-Miller, Taylor, Bibb: "Integrated regulation of PKA by fast and slow neurotransmission in the nucleus accumbens controls plasticity and stress responses." in: The Journal of biological chemistry, Vol. 298, Issue 8, pp. 102245, (2022) (PubMed).

Gupta, Strange, Telange, Guo, Hatch, Sobh, Elie, Carter, Totenhagen, Tan, Sonawane, Neuzil, Natarajan, Ovens, Oakhill, Wiederhold, Pacak, Ghayee, Meijer, Reddy, Bibb: "Genetic impairment of succinate metabolism disrupts bioenergetic sensing in adrenal neuroendocrine cancer." in: Cell reports, Vol. 40, Issue 7, pp. 111218, (2022) (PubMed).

Carter, Kumar, Herring, Tan, Guenter, Telange, Howse, Viol, McCaw, Bickerton, Gupta, Gillardon, Woltering, Dhall, Totenhagen, Banerjee, Kurian, Reddy, Chen, Schrader, Bart Rose, Mukhtar, Bibb: "Cdk5 drives formation of heterogeneous pancreatic neuroendocrine tumors." in: Oncogenesis, Vol. 10, Issue 12, pp. 83, (2021) (PubMed).

Target Details for CDK5

Target: CDK5 (Cyclin-Dependent Kinase 5 (CDK5))

Alternative Name: CDK5

Background:

Synonyms: mouse anti-cdk5 antibody, cdk, mouse anti-Cyclin-dependent kinase 5, Cell division protein kinase 5, Serine/threonine-protein kinase PSSALRE, Tau protein kinase II catalytic subunit

Background: CDK5 antibody recognizes the neuronal protein kinase Cdk5 which is implicated in a vast array of normal neuronal functions including regulation of neurotransmitter synthesis, the presynaptic vesicle cycle, neurotransmitter receptor trafficking and dopamine neurotransmission. At the same time Cdk5 has been implicated in a plethora of neurological and neuropsychiatric disorders including Alzheimer's, Parkinson's, Huntington's, epilepsy, schizophrenia, and drug addiction. Detection of Cdk5 in normal samples as well as tissue undergoing neurodegeneration may advance studies in these areas. Moreover, this antibody may allow more accurate postmortem evaluations of Cdk5 protein expression, and thus serve as a valuable new reagent for neuropathology. Anti-CDK5 antibody is ideal for investigators involved in Cell Signaling, Neuroscience, Signal Transduction research.

Gene Name: CDK5

Gene ID: 140908

UniProt: Q03114

Pathways: Cell Division Cycle, Regulation of Muscle Cell Differentiation, Synaptic Membrane, Regulation of Cell Size, Skeletal Muscle Fiber Development, Synaptic Vesicle Exocytosis