GRIN2B antibody (N-Term)

Catalog No. ABIN6658131

Supplier Product No.: 200-301-g23

This Mouse Monoclonal antibody specifically detects GRIN2B in WB, IHC, IP, IF and FM. It exhibits reactivity toward Rat.

Quick Overview for GRIN2B antibody (N-Term) (ABIN6658131)

Target: GRIN2B (Glutamate Receptor, Ionotropic, N-Methyl D-Aspartate 2B (GRIN2B))

Reactivity: Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: This GRIN2B antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunofluorescence (IF), Fluorescence Microscopy (FM)

Clone: S59-36

Product Details anti-GRIN2B Antibody

Binding Specificity: N-Term

Supplier Product No.: 200-301-g23

Supplier: Rockland

Purpose: NR2B Antibody

Cross-Reactivity (Details): A BLAST analysis was used to suggest cross-reactivity with NR2B from human, mouse, and rat based on 100 % homology with the immunizing sequence.

Purification: Anti-NR2B Antibody was purified by Protein G chromatography.

Sterility: Sterile filtered

Immunogen: NR2B Antibody was produced in mice by repeated immunizations raised against a fusion protein corresponding near the extracellular N-terminus region of rat NR2B.

Isotype: IgG2b

Application Details

Application Notes:

Immunoprecipitation_Dilution: User Optimized

Immunohistochemistry_Dilution: 0.1-1.0 μg/mL

IF_Microscopy_Dilution: 1.0-10 μg/mL

Western_Blot_Dilution: 1-10 μg/mL

Comment:

Anti-NR2B Antibody is tested for use in WB, IHC, IF, and IP. Expect a band approximately ~166kDa on specific lysates. Specific conditions for reactivity should be optimized by the end user.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 50 % (v/v) Glycerol

Storage: 4 °C,-20 °C

Storage Comment: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Expiry Date: 12 months

Target Details for GRIN2B

Target: GRIN2B (Glutamate Receptor, Ionotropic, N-Methyl D-Aspartate 2B (GRIN2B))

Alternative Name: NR2B

Background:

Synonyms: NMDAR2B, hNR3, MGC142180, glutamate receptor subunit epsilon-2, NR3, NR2B, N-methyl D-aspartate receptor subtype 2B

Background: NR2B containing receptors have been implicated in synaptic plasticity, memory formation and pain modulation. Studies suggest that the NR2B subunit of glutamate receptors may be potential targets for relieving pain, NR2B may be a probable target for anti-nociceptive drugs, and may also be useful as analgesics.

Gene Name: Grin2b

Gene ID: 24410

NCBI Accession: NP_036706

UniProt: Q00960

Pathways: Response to Growth Hormone Stimulus, Synaptic Membrane, Feeding Behaviour, Regulation of long-term Neuronal Synaptic Plasticity