SLC32A1 antibody (N-Term)

Catalog No. ABIN6658150

Product Details anti-SLC32A1 Antibody

Target: SLC32A1 (Solute Carrier Family 32 (GABA Vesicular Transporter), Member 1 (SLC32A1))

Binding Specificity: N-Term

Reactivity: Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SLC32A1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Cross-Reactivity: Chicken, Mouse (Murine), Rat (Rattus)

Cross-Reactivity (Details): Cross reactivity with VGAT from other species has not been determined.

Purification: Anti-Vesicular GABA transporter antibody is affinity purified from monospecific antiserum by immunoaffinity purification. This antibody is directed against rat VGAT. Expect reactivity with the following species based on sequence homology: bovine, canine, non-human primate.

Immunogen:

Immunogen: Anti-Vesicular GABA transporter Antibody was produced in rabbit by repeated immunizations with synthetic peptide corresponding to amino acid residues from the N-terminal region conjugated to KLH.

Immunogen Type: Peptide

Isotype: IgG

Application Details

Application Notes:

Application Note: Anti-VGAT antibody is suitable for use in ELISA and Western Blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band of approximately 53 kDa in size corresponding to vesicular GABA transporter protein in the appropriate cell lysate or extract.

ELISA Dilution: 1:10,000

Western Blot Dilution: 1:1000

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer:

Buffer: 0.01 M HEPES, 0.15 M Sodium Chloride, pH 7.5

Stabilizer: 0.1 mg/mL Bovine Serum Albumin (BSA) - IgG and Protease free, 50 % (v/v) Glycerol

Storage: 4 °C,-20 °C

Storage Comment: Store vial at -20° C prior to opening.  This product is stable at 4° C as an undiluted liquid.  For extended storage, aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing. Dilute only prior to immediate use.

Target Details for SLC32A1

Target: SLC32A1 (Solute Carrier Family 32 (GABA Vesicular Transporter), Member 1 (SLC32A1))

Alternative Name: Vesicular GABA transporter (VGAT) (SLC32A1 Products)

Synonyms: VGAT antibody, VIAAT antibody, vGAT antibody, Ci-vGAT antibody, slc32a1 antibody, viaat antibody, MGC68938 antibody, vgat antibody, xVIAAT antibody, R75019 antibody, Viaat antibody, CG8394 antibody, CG8394.2 antibody, Dmel\\CG8394 antibody, Vgat antibody, zgc:158324 antibody, solute carrier family 32 member 1 antibody, vesicular GABA transporter antibody, solute carrier family 32 member 1 S homeolog antibody, vesicular inhibitory amino acid transporter antibody, Vesicular inhibitory amino acid transporter antibody, solute carrier family 32 (GABA vesicular transporter), member 1 antibody, Vesicular GABA Transporter antibody, SLC32A1 antibody, vgat antibody, Bm1_20950 antibody, slc32a1.S antibody, slc32a1 antibody, CpipJ_CPIJ002704 antibody, viaat antibody, Slc32a1 antibody, VGAT antibody, Tsp_04599 antibody

Background:

Synonyms: Vesicular inhibitory amino acid transporter, GABA and glycine transporter, Solute carrier family 32 member 1, Vesicular GABA transporter, rGVAT

Background: Vesicular GABA transporter antibody recognizes Vesicular GABA Amino Acid Transporter (VGAT) which is responsible for transport of the inhibitory neurotransmitter into synaptic vesicles. The VGAT protein (also known as the Vesicular Inhibitory Amino Aid Transporter or VIAAT) is expressed in synaptic vesicles of both glycine and GABAergic synapses throughout the CNS. Expression of the VGAT protein changes during development and also in response to patterns of neuronal activity. Therefore, Vesicular GABA transporter antibody is ideal for investigators involved in Neuronal Circuitry and, more generally in Neuroscience.

Gene Name: SLC32A1

Gene ID: 83612

UniProt: O35458

Pathways: Proton Transport