GRIN2B antibody (pTyr1336)

Catalog No. ABIN6658156

Supplier Product No.: 612-401-c88

The Rabbit Polyclonal anti-GRIN2B antibody has been validated for WB and IHC. It is suitable to detect GRIN2B in samples from Rat.

Quick Overview for GRIN2B antibody (pTyr1336) (ABIN6658156)

Target: GRIN2B (Glutamate Receptor, Ionotropic, N-Methyl D-Aspartate 2B (GRIN2B))

Reactivity: Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This GRIN2B antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-GRIN2B Antibody

Binding Specificity: pTyr1336

Supplier Product No.: 612-401-c88

Supplier: Rockland

Purpose: NMDA NR2B Subunit phospho Y1336 Antibody

Cross-Reactivity (Details): Anti-NMDA NR2B Subunit pY1336 antibody is directed against rat NMDA 2B receptor protein.

Purification: The antibody was affinity purified from monospecific antiserum by immunoaffinity purification.

Immunogen: Anti-NMDA NR2B Subunit pY1336 Antibody was produced by repeated immunizations with a synthetic phospho-peptide corresponding to amino acid residues surrounding Tyr1336.

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry_Dilution: 1:400

Western_Blot_Dilution: 1:1000

Other: User Optimized

Comment:

Anti-NMDA NR2B Subunit pY1336 (Rabbit) antibody is tested for use in Western Blotting and IHC. Specific conditions for reactivity should be optimized by the end user. Expect a band of approximately 180 kDa in size corresponding to NMDAR NR2B subunit protein phosphorylated at Tyr1336 in the appropriate cell lysate or extract.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer:

Buffer: 0.01 M HEPES, 0.15 M Sodium Chloride, pH 7.5

Stabilizer: 0.1 mg/mL Bovine Serum Albumin (BSA) - IgG and Protease free, 50 % (v/v) Glycerol

Storage: 4 °C,-20 °C

Storage Comment: Store vial at -20° C prior to opening. This product is stable at 4° C as an undiluted liquid. For extended storage, aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Dilute only prior to immediate use.

Expiry Date: 12 months

Target Details for GRIN2B

Target: GRIN2B (Glutamate Receptor, Ionotropic, N-Methyl D-Aspartate 2B (GRIN2B))

Alternative Name: NMDA NR2B Subunit

Background:

Synonyms: Glutamate [NMDA] receptor subunit epsilon-2, N-methyl D-aspartate receptor subtype 2B, NMDAR2B, NR2B, Grin2b

Background: NMDA 2B pY1336 antibody detects NMDA 2B receptor protein. The NMDAR plays an essential role in memory, neuronal development and it has also been implicated in several disorders of the central nervous system including Alzheimer's, epilepsy and ischemic neuronal cell death. The rat NMDAR1 (NR1) was the first subunit of the NMDAR to be cloned. The NR1 protein can form NMDA activated channels when expressed in Xenopus oocytes but the currents in such channels are much smaller than those seen in situ. Channels with more physiological characteristics are produced when the NR1 subunit is combined with one or more of the NMDAR2 (NR2 A-D) subunits. Phosphorylation of Tyr1336 is thought to potentiate NMDA receptor-dependent influx of calcium and ischemia may also increase the phosphorylation of this site. NMDA NR2B Subunit pY1336 antibody is ideal for investigators involved in Cell Signaling, Neuroscience, and Signal Transduction research.

Gene Name: GRIN2B

Gene ID: 24410

NCBI Accession: NP_036706

UniProt: Q00960

Pathways: Response to Growth Hormone Stimulus, Synaptic Membrane, Feeding Behaviour, Regulation of long-term Neuronal Synaptic Plasticity