GRIN2B antibody (pTyr1472)

Catalog No. ABIN6658157

Supplier Product No.: 612-401-c89

This anti-GRIN2B antibody is a Rabbit Polyclonal antibody detecting GRIN2B in WB. Suitable for Rat. This Primary Antibody has been cited in 1 publication.

Quick Overview for GRIN2B antibody (pTyr1472) (ABIN6658157)

Target: GRIN2B (Glutamate Receptor, Ionotropic, N-Methyl D-Aspartate 2B (GRIN2B))

Reactivity: Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This GRIN2B antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-GRIN2B Antibody

Binding Specificity: pTyr1472

Supplier Product No.: 612-401-c89

Supplier: Rockland

Purpose: NMDA NR2B Subunit phospho Y1472 Antibody

Cross-Reactivity (Details): Anti-NMDA NR2B Subunit pY1472 antibody is directed against rat NMDA NR2B receptor protein.

Purification: The antibody was affinity purified from monospecific antiserum by immunoaffinity purification.

Immunogen: Anti-NMDA NR2B Subunit pY1472 Antibody was produced by repeated immunizations with synthetic phospho-peptide corresponding to amino acid residues surrounding Tyr1472.

Isotype: IgG

Application Details

Application Notes:

Western_Blot_Dilution: 1:1000

Other: User Optimized

Comment:

Anti-NMDA NR2B Subunit pY1472 (Rabbit) antibody is tested for use in Western Blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band of approximately 180 kDa in size corresponding to NMDAR NR2B subunit protein phosphorylated at Tyr1472 in the appropriate cell lysate or extract. This antibody also labels proteins of approximately 65 kDa and 115 kDa in size.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer:

Buffer: 0.01 M HEPES, 0.15 M Sodium Chloride, pH 7.5

Stabilizer: 0.1 mg/mL Bovine Serum Albumin (BSA) - IgG and Protease free, 50 % (v/v) Glycerol

Storage: 4 °C,-20 °C

Storage Comment: Store vial at -20° C prior to opening. This product is stable at 4° C as an undiluted liquid. For extended storage, aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Dilute only prior to immediate use.

Expiry Date: 12 months

References for anti-GRIN2B antibody (ABIN6658157)

Gonda, Giesen, Sieberath, West, Buchholz, Klatt, Ziebarth, Räk, Kleinhubbert, Riedel, Hollmann, Hamad, Reiner, Wahle: "GluN2B but Not GluN2A for Basal Dendritic Growth of Cortical Pyramidal Neurons." in: Frontiers in neuroanatomy, Vol. 14, pp. 571351, (2020) (PubMed).

Target Details for GRIN2B

Target: GRIN2B (Glutamate Receptor, Ionotropic, N-Methyl D-Aspartate 2B (GRIN2B))

Alternative Name: NMDA NR2B Subunit

Background:

Synonyms: Glutamate [NMDA] receptor subunit epsilon-2, N-methyl D-aspartate receptor subtype 2B, NMDAR2B, NR2B, Grin2b, NR3

Background: NMDA NR2B Subunit pY1472 antibody detects NMDA NR2B receptor protein. The ion channels activated by glutamate that are sensitive to N-methyl-D-aspartate (NMDA) are designated NMDA receptors (NMDAR). The NMDAR plays an essential role in memory, neuronal development and it has also been implicated in several disorders of the central nervous system including Alzheimer's, epilepsy and ischemic neuronal cell death. The NMDA receptor is also one of the principal molecular targets for alcohol in the CNS. Channels with physiological characteristics are produced when the NR1 subunit is combined with one or more of the NMDAR2 (NR2 A-D) subunits. Overexpression of the NR2B-subunit of the NMDA Receptor has been associated with increases in learning and memory while aged, memory impaired animals have deficiencies in NR2B expression. Recent work suggests that phosphorylation of Tyr1472 on NR2B may regulate the functional expression the receptor in LTP and other forms of plasticity. NMDA NR2B Subunit pY1472 antibody is ideal for investigators involved in Cell Signaling, Neuroscience, and Signal Transduction research.

Gene Name: GRIN2B

Gene ID: 24410

NCBI Accession: NP_036706

UniProt: Q00960

Pathways: Response to Growth Hormone Stimulus, Synaptic Membrane, Feeding Behaviour, Regulation of long-term Neuronal Synaptic Plasticity