Metabotropic Glutamate Receptor 5 antibody (C-Term)

Catalog No. ABIN6658205

Supplier Product No.: 612-401-d77

This anti-Metabotropic Glutamate Receptor 5 antibody is a Rabbit Polyclonal antibody detecting Metabotropic Glutamate Receptor 5 in WB and IHC. Suitable for Rat.

Quick Overview for Metabotropic Glutamate Receptor 5 antibody (C-Term) (ABIN6658205)

Target: Metabotropic Glutamate Receptor 5 (GRM5) (Glutamate Receptor, Metabotropic 5 (GRM5))

Reactivity: Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Metabotropic Glutamate Receptor 5 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-Metabotropic Glutamate Receptor 5 Antibody

Binding Specificity: C-Term

Supplier Product No.: 612-401-d77

Supplier: Rockland

Purpose: mGluR5/1a Antibody

Cross-Reactivity (Details): Anti-mGluR5/1a antibody is directed against GluR5/1a.

Purification: GluR5/1a antibody was affinity purified from monospecific antiserum by immunoaffinity purification.

Immunogen: Anti-mGluR5/1a Antibody was produced in rabbit by repeated immunizations with synthetic peptide corresponding to amino acid residues from the C-terminal region conjugated to KLH.

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry_Dilution: 1:500

Western_Blot_Dilution: 1:1000

Comment:

Anti-mGluR5/1a (Rabbit) antibody is tested for use in Western Blotting and IHC. Specific conditions for reactivity should be optimized by the end user. Expect a band of approximately 125 kDa monomer and 250 kDa dimer in size corresponding to mGluR5 and mGluR1 proteins in the appropriate cell lysate or extract.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer:

Buffer: 0.01 M HEPES, 0.15 M Sodium Chloride, pH 7.5

Stabilizer: 0.1 mg/mL Bovine Serum Albumin (BSA) - IgG and Protease free, 50 % (v/v) Glycerol

Storage: 4 °C,-20 °C

Storage Comment: Store vial at -20° C prior to opening.  This product is stable at 4° C as an undiluted liquid.  For extended storage, aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing. Dilute only prior to immediate use.

Expiry Date: 12 months

Target Details for Metabotropic Glutamate Receptor 5

Target: Metabotropic Glutamate Receptor 5 (GRM5) (Glutamate Receptor, Metabotropic 5 (GRM5))

Alternative Name: Grm5

Background:

Synonyms: mGluR5, Metabotropic Glutamate Receptor 5/1a

Background: mGluR5/1a antibody detects GluR5/1a protein. The metabotropic glutamate receptors (mGluRs) are key receptors in the modulation of excitatory synaptic transmission in the central nervous system. They are implicated in many forms of neural plasticity as well as learning and memory and drug abuse. Group I metabotropic glutamate receptors (consisting of mGluR1 and mGluR5) are G-protein-coupled neurotransmitter receptors that are localized in the perisynaptic region of the postsynaptic membrane. When activated, Group I mGluRs lead to stimulation of phospholipase and activation of Protein Kinase C. In contrast, activation of Group II metabotropic receptors (mGluR2 and mGluR3) leads to inhibition of adenylate cyclase. The mGluR1 receptor may also be critically involved in limiting the deleterious effects of excitotoxicity. In contrast, the mGluR5 receptor appears to be essential for late phase LTP in area CA1 of the hippocampus. mGluR5/1a is ideal for investigators involved neuronal circuitry and more generally in Neuroscience.

Gene Name: GRM5

Gene ID: 24418

UniProt: P31424

Pathways: Regulation of G-Protein Coupled Receptor Protein Signaling, Regulation of long-term Neuronal Synaptic Plasticity