MAPT antibody (pSer416)

Catalog No. ABIN6658211

Supplier Product No.: 612-401-e42

This anti-MAPT antibody is a Rabbit Polyclonal antibody detecting MAPT in WB and IHC. Suitable for Rat.

Quick Overview for MAPT antibody (pSer416) (ABIN6658211)

Target: MAPT (Microtubule-Associated Protein tau (MAPT))

Reactivity: Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MAPT antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-MAPT Antibody

Binding Specificity: pSer416

Supplier Product No.: 612-401-e42

Supplier: Rockland

Purpose: Tau phospho S416 Antibody

Cross-Reactivity (Details): Anti-Tau pS416 antibody is directed against rat Tau.

Purification: Tau pS416 antibodies was prepared from monospecific antiserum by immunoaffinity chromatography using phospho peptide coupled to agarose beads followed by solid phase adsorption(s) against non-phospho peptide and non-specific peptide to remove any unwanted reactivities.

Immunogen: Anti-Tau pS416 Antibody was produced in rabbit by repeated immunizations with synthetic phospho-peptide corresponding to amino acid residues surrounding Ser416 of the Tau protein.

Isotype: IgG

Application Details

Application Notes: Optional[Neutralization_Dilution]: 1:1000

Comment:

Anti-Tau pS416 (Rabbit) antibody is tested for use in Western Blot and IHC. Expect bands approximately 59, 65, 68 kDa in size corresponding to tau proteins phosphorylated at Ser416 in the appropriate cell lysate or extract. Researchers should determine optimal titers for applications that are not stated below.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer:

Buffer: 0.01 M HEPES, 0.15 M Sodium Chloride, pH 7.5

Stabilizer: 0.1 mg/mL Bovine Serum Albumin (BSA) - IgG and Protease free, 50 % (v/v) Glycerol

Storage: 4 °C,-20 °C

Storage Comment: Store vial at -20° C prior to opening.  This product is stable at 4° C as an undiluted liquid.  For extended storage, aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing. Dilute only prior to immediate use.

Expiry Date: 12 months

Target Details for MAPT

Target: MAPT (Microtubule-Associated Protein tau (MAPT))

Alternative Name: Mapt

Background:

Synonyms: Microtubule-associated protein tau, Neurofibrillary tangle protein, Paired helical filament-tau, PHF-tau

Background: Tau pS416 Antibody detects Tau, which s a key microtubule-associated protein that plays an important role in the formation of microtubules in axons. Six tau isoforms have been identified as products of a single gene produced by alternative mRNA splicing. Tau mutations have been implicated in many neurodegenerative disorders such as Alzheimer's disease (AD), Pick's disease and progressive supranuclear palsy. It has been well documented that hyperphosphorylated tau is a major component of paired helical filaments in AD brain. Serine 416 has been demonstrated to be a major phosphorylation site in vitro by CaM kinase II. Anti-Tau pS416 Antibody is ideal for investigators involved in Cell Signaling, Neuroscience, Signal Transduction research.

Gene Name: MAPT

Gene ID: 69329

UniProt: P19332

Pathways: MAPK Signaling, Microtubule Dynamics, M Phase, Regulation of Cell Size