GABRA2 antibody (Cytoplasmic Loop)

Catalog No. ABIN6658231

Supplier Product No.: 600-401-d45

There are 2+ publications for this product available. The Rabbit Polyclonal anti-GABRA2 antibody is suitable to detect GABRA2 in samples from Rat. It has been validated for WB.

Quick Overview for GABRA2 antibody (Cytoplasmic Loop) (ABIN6658231)

Target: GABRA2 (gamma-aminobutyric Acid (GABA) A Receptor, alpha 2 (GABRA2))

Reactivity: Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This GABRA2 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-GABRA2 Antibody

Binding Specificity: Cytoplasmic Loop

Supplier Product No.: 600-401-d45

Supplier: Rockland

Purpose: GABA(A) Receptor alpha 2 Antibody

Cross-Reactivity (Details): Anti-GABA(A) Receptor alpha 2 antibody is directed against rat GABA(A) receptor alpha 2 protein.

Purification: The antibody was affinity purified from monospecific antiserum by immunoaffinity purification.

Immunogen: Anti-GABA(A) Receptor alpha 2 Antibody was produced in rabbit by repeated immunizations with a fusion protein from the cytoplasmic loop of the rat receptor alpha 2 subunit.

Isotype: IgG

Application Details

Application Notes: Optional[Neutralization_Dilution]: 1:1000

Comment:

Suggested Applications: IF
Anti-GABA(A) Receptor alpha 2 (Rabbit) Antibody is tested for use in Western Blot and ICC. Specific conditions for reactivity should be optimized by the end user. Expect a band of approximately 51 kDa in size corresponding to the alpha 2 subunit of the GABA(A) receptor in the appropriate cell lysate or extract.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer:

Buffer: 0.01 M HEPES, 0.15 M Sodium Chloride, pH 7.5

Stabilizer: 0.1 mg/mL Bovine Serum Albumin (BSA) - IgG and Protease free, 50 % (v/v) Glycerol

Storage: 4 °C,-20 °C

Storage Comment: Store vial at -20° C prior to opening.  This product is stable at 4° C as an undiluted liquid.  For extended storage, aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing. Dilute only prior to immediate use.

Expiry Date: 12 months

References for anti-GABRA2 antibody (ABIN6658231)

Kreye, Wright, van Casteren, Stöffler, Machule, Reincke, Nikolaus, van Hoof, Sanchez-Sendin, Homeyer, Cordero Gómez, Kornau, Schmitz, Kaindl, Boehm-Sturm, Mueller, Wilson, Upadhya, Dhangar, Greenhill, Woodhall, Turko, Vida, Garner, Wickel, Geis, Fukata, F: "Encephalitis patient-derived monoclonal GABAA receptor antibodies cause epileptic seizures." in: The Journal of experimental medicine, Vol. 218, Issue 11, (2021) (PubMed).

Ohkawa, Satake, Yokoi, Miyazaki, Ohshita, Sobue, Takashima, Watanabe, Fukata, Fukata: "Identification and characterization of GABA(A) receptor autoantibodies in autoimmune encephalitis." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 34, Issue 24, pp. 8151-63, (2014) (PubMed).

Target Details for GABRA2

Target: GABRA2 (gamma-aminobutyric Acid (GABA) A Receptor, alpha 2 (GABRA2))

Alternative Name: GABA(A) Receptor alpha 2

Background:

Synonyms: Gamma-aminobutyric acid receptor subunit alpha-2, GABA(A) receptor subunit alpha-2

Background: GABA(A) Receptor alpha 2 Antibody detects GABA(A) receptor alpha 2 protein. Gamma-aminobutyric acid (GABA) is the primary inhibitory neurotransmitter in the central nervous system, causing a hyperpolarization of the membrane through the opening of a Cl- channel associated with the GABAA receptor (GABAA-R) subtype. GABAA-Rs are important therapeutic targets for a range of sedative, anxiolytic, and hypnotic agents and are implicated in several diseases including epilepsy, anxiety, depression, and substance abuse. The GABAA-R is a multimeric subunit complex. To date six αs, four βs and four γs, plus alternative splicing variants of some of these subunits, have been identified. Injection in oocytes or mammalian cell lines of cRNA coding for α- and β-subunits results in the expression of functional GABAA-Rs sensitive to GABA. However, coexpression of a γ-subunit is required for benzodiazepine modulation. The various effects of the benzodiazepines in brain may also be mediated via different α-subunits of the receptor. GABA(A) receptor alpha 2 antibody is ideal for investigators involved in Neuroscience.

Gene Name: GABRA2

Gene ID: 61856

NCBI Accession: NP_001129251

UniProt: P23576